Skip to content

Research at St Andrews

Partial purification of antibacterial proteinaceous factors from erythrocytes of Oncorhynchus mykiss

Research output: Contribution to journalArticle

Author(s)

J M O Fernandes, V J Smith

School/Research organisations

Abstract

Antimicrobial peptides are natural antibiotics known to be present in both myeloid cells and epithelial surfaces of vertebrates. Nevertheless, the reports of antimicrobial peptides isolated from blood cells of teleosts are scarce. In this paper we show that acid-soluble erythrocyte extracts from rainbow trout, Oncorhynchus mykiss, display antibacterial activity against Planococcus citreus on a radial diffusion assay. Following C-t(18) solid phase extraction, cationic exchange chromatography and C-18 reversed phase HPLC, two groups of fractions with antibacterial properties were obtained. This antibacterial activity is thermostable and susceptible to digestion by proteinase K, thus showing that the antibacterial agents have a proteinaceous nature. The factors eluted from a C-18 column with circa 33% acetonitrile are active against P. citreus and Escherichia coli, with minimal inhibitory concentrations in the range 7-14 mug ml(-1) and 14-28 mug ml(-1), respectively; the ones eluted with approximately 44% acetonitrile on the same column only displayed activity against P. citreus, with a minimal inhibitory concentration of 1-2 mug ml(-1). These results raise the possibility that trout erythrocytes may contain antimicrobial factors not previously considered to be part of the innate immune system. (C) 2003 Elsevier Ltd. All rights reserved.

Close

Details

Original languageEnglish
Pages (from-to)19
Number of pages9
JournalFish and Fisheries
Volume16
DOIs
Publication statusPublished - Jan 2004

    Research areas

  • antibacterial protein, trout, Oncorhynchus mykiss, erythrocytes, blood, ANTIMICROBIAL PEPTIDE, RAINBOW-TROUT, SKIN SECRETIONS, HISTONE H2A, FISH, PLEUROCIDIN, ANTIBIOTICS, FLOUNDER, SALMON, BASS

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Cellular stress responses to chronic heat shock and shell damage in temperate Mya truncata

    Sleight, V. A., Peck, L. S., Dyrynda, E. A., Smith, V. J. & Clark, M. S., 12 May 2018, In : Cell Stress and Chaperones. First Online, 15 p.

    Research output: Contribution to journalArticle

  2. Wind turbines cause chronic stress in badgers (Meles meles) in Great Britain

    Agnew, R., Smith, V. J. & Fowkes, R., Jul 2016, In : Journal of Wildlife Diseases. 52, 3, p. 459-467 9 p.

    Research output: Contribution to journalArticle

  3. Hematopoiesis and hemocytes in pancrustacean and molluscan models

    Smith, V. J., Accorsi, A. & Malagoli, D., 20 May 2016, The Evolution of the Immune System: Conservation and Diversification. Malagoli, D. (ed.). 1st ed. Academic Press/Elsevier , p. 1-28

    Research output: Chapter in Book/Report/Conference proceedingChapter

  4. Immunology of invertebrates: Cellular

    Smith, V. J., 15 Feb 2016, Encyclopedia of Life Sciences: Wiley. Wiley, 13 p. (Encyclopedia of Life Sciences).

    Research output: Chapter in Book/Report/Conference proceedingChapter

Related by journal

  1. The remarkable reproductive diversity of teleost fishes

    Smith, C. & Wootton, R. J., Dec 2016, In : Fish and Fisheries. 17, 4, p. 1208-1215 8 p.

    Research output: Contribution to journalArticle

  2. Biology of the three-spined stickleback.

    Smith, C., 2009, In : Fish and Fisheries. 10, p. 354-355.

    Research output: Contribution to journalArticle

  3. Sensuous seas

    Smith, C., 2008, In : Fish and Fisheries. 9, p. 217-218

    Research output: Contribution to journalArticle

  4. The introduction of the European bitterling (Rhodeus amarus) to West and Central Europe

    Van Damme, D., Bogutskaya, N., Hoffman, RC. & Smith, C. H., 2007, In : Fish and Fisheries. 8, p. 79-106

    Research output: Contribution to journalArticle

  5. Are stock assessment methods too complicated?

    Cotter, AJR., Burt, M. L., Paxton, CGM., Fernández, C., Buckland, S. T. & Pan, J-X., Sep 2004, In : Fish and Fisheries. 5, p. 235-254 20 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Fish and Fisheries (Journal)

    Kevin Neville Laland (Editor)
    2003

    Activity: Publication peer-review and editorial work typesEditor of research journal

ID: 735575