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PDF cells are a GABA-responsive wake-promoting component of the Drosophila sleep circuit

Research output: Contribution to journalArticle

Author(s)

Katherine M Parisky, Jose Agosto, Stefan R Pulver, Yuhua Shang, Elena Kuklin, James J L Hodge, Kyeongjin Kang, Keongjin Kang, Xu Liu, Paul A Garrity, Michael Rosbash, Leslie C Griffith

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Abstract

Daily sleep cycles in humans are driven by a complex circuit within which GABAergic sleep-promoting neurons oppose arousal. Drosophila sleep has recently been shown to be controlled by GABA, which acts on unknown cells expressing the Rdl GABAA receptor. We identify here the relevant Rdl-containing cells as PDF-expressing small and large ventral lateral neurons (LNvs) of the circadian clock. LNv activity regulates total sleep as well as the rate of sleep onset; both large and small LNvs are part of the sleep circuit. Flies mutant for pdf or its receptor are hypersomnolent, and PDF acts on the LNvs themselves to control sleep. These features of the Drosophila sleep circuit, GABAergic control of onset and maintenance as well as peptidergic control of arousal, support the idea that features of sleep-circuit architecture as well as the mechanisms governing the behavioral transitions between sleep and wake are conserved between mammals and insects.
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Details

Original languageEnglish
Pages (from-to)672-82
Number of pages11
JournalNeuron
Volume60
Issue number4
DOIs
Publication statusPublished - 26 Nov 2008

    Research areas

  • Animals, Arousal, Biological Evolution, Brain, Circadian Rhythm, Drosophila Proteins, Drosophila melanogaster, Mammals, Molecular Biology, Neural Pathways, Neurons, Neuropeptides, Sleep, Species Specificity, Synaptic Transmission, TRPC Cation Channels, Wakefulness, gamma-Aminobutyric Acid

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