Skip to content

Research at St Andrews

Phylogeographic variation in recombination rates within a global clone of methicillin-resistant Staphylococcus aureus

Research output: Contribution to journalArticle

Author(s)

Santiago Castillo-Ramirez, Jukka Corander, Pekka Marttinen, Mona Aldeljawi, William P. Hanage, Henrik Westh, Kit Boye, Zeynep Gulay, Stephen D. Bentley, Julian Parkhill, Matthew T. Holden, Edward J. Feil

School/Research organisations

Abstract

Background: Next-generation sequencing (NGS) is a powerful tool for understanding both patterns of descent over time and space (phylogeography) and the molecular processes underpinning genome divergence in pathogenic bacteria. Here, we describe a synthesis between these perspectives by employing a recently developed Bayesian approach, BRATNextGen, for detecting recombination on an expanded NGS dataset of the globally disseminated methicillin-resistant Staphylococcus aureus (MRSA) clone ST239.

Results: The data confirm strong geographical clustering at continental, national and city scales and demonstrate that the rate of recombination varies significantly between phylogeographic sub-groups representing independent introductions from Europe. These differences are most striking when mobile non-core genes are included, but remain apparent even when only considering the stable core genome. The monophyletic ST239 sub-group corresponding to isolates from South America shows heightened recombination, the sub-group predominantly from Asia shows an intermediate level, and a very low level of recombination is noted in a third sub-group representing a large collection from Turkey.

Conclusions: We show that the rapid global dissemination of a single pathogenic bacterial clone results in local variation in measured recombination rates. Possible explanatory variables include the size and time since emergence of each defined sub-population (as determined by the sampling frame), variation in transmission dynamics due to host movement, and changes in the bacterial genome affecting the propensity for recombination.

Close

Details

Original languageEnglish
Article number126
Number of pages13
JournalGenome Biology
Volume13
Issue number12
Publication statusPublished - 2012

    Research areas

  • SEQUENCE TYPE, EVOLUTION, TRANSMISSION, MRSA, OUTBREAK, BACTERIA, SPREAD

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Genomic identification of cryptic susceptibility to penicillins and β-lactamase inhibitors in methicillin-resistant Staphylococcus aureus

    Harrison, E. M., Ba, X., Coll, F., Blane, B., Restif, O., Carvell, H., Köser, C. U., Jamrozy, D., Reuter, S., Lovering, A., Gleadall, N., Bellis, K. L., Uhlemann, A. C., Lowy, F. D., Massey, R. C., Grilo, I. R., Sobral, R., Larsen, J., Rhod Larsen, A., Vingsbo Lundberg, C. & 5 othersParkhill, J., Paterson, G. K., Holden, M. T. G., Peacock, S. J. & Holmes, M. A., 24 Jun 2019, In : Nature Microbiology. 15 p.

    Research output: Contribution to journalArticle

  2. Atlas of group A streptococcal vaccine candidates compiled using large-scale comparative genomics

    Davies, M. R., McIntyre, L., Mutreja, A., Lacey, J. A., Lees, J. A., Towers, R. J., Duchêne, S., Smeesters, P. R., Frost, H. R., Price, D. J., Holden, M. T. G., David, S., Giffard, P. M., Worthing, K. A., Seale, A. C., Berkley, J. A., Harris, S. R., Rivera-Hernandez, T., Berking, O., Cork, A. J. & 18 othersTorres, R. S. L. A., Lithgow, T., Strugnell, R. A., Bergmann, R., Nitsche-Schmitz, P., Chhatwal, G. S., Bentley, S. D., Fraser, J. D., Moreland, N. J., Carapetis, J. R., Steer, A. C., Parkhill, J., Saul, A., Williamson, D. A., Currie, B. J., Tong, S. Y. C., Dougan, G. & Walker, M. J., Jun 2019, In : Nature Genetics. 51, 6, p. 1035-1043

    Research output: Contribution to journalArticle

  3. Antimicrobial resistance in atopic dermatitis: need for an urgent rethink

    Harkins, C. P., Holden, M. T. G. & Irvine, A. D., Mar 2019, In : Annals of Allergy Asthma & Immunology. 122, 3, p. 236-240 5 p.

    Research output: Contribution to journalArticle

  4. Molecular epidemiology and expression of capsular polysaccharides in Staphylococcus aureus clinical isolates in the United States

    Mohamed, N., Timofeyeva, Y., Jamrozy, D., Rojas, E., Hao, L., Silmon de Monerri, N. C., Hawkins, J., Singh, G., Cai, B., Liberator, P., Sebastian, S., Donald, R. G. K., Scully, I. L., Jones, C. H., Creech, C. B., Thomsen, I., Parkhill, J., Peacock, S. J., Jansen, K. U., Holden, M. T. G. & 1 othersAnderson, A. S., 14 Jan 2019, In : PLoS ONE. 14, 1, 33 p., e0208356.

    Research output: Contribution to journalArticle

Related by journal

  1. Wnt evolution and function shuffling in liberal and conservative chordate genomes

    Somorjai, I. M. L., Marti-Solans, J., Diaz-Gracia, M., Nishida, H., Imai, K., Escriva, H., Cañestro, C. & Albalat, R., 25 Jul 2018, In : Genome Biology. 19, 17 p., 98.

    Research output: Contribution to journalArticle

  2. Methicillin-resistant Staphylococcus aureus emerged long before the introduction of methicillin into clinical practice

    Harkins, C. P., Pichon, B., Doumith, M., Parkhill, J., Westh, H., Tomasz, A., de Lencastre, H., Bentley, S. D., Kearns, A. M. & Holden, M. T. G., 20 Jul 2017, In : Genome Biology. 18, 11 p., 130.

    Research output: Contribution to journalArticle

  3. The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the basis of parasitism and virulence

    Eves-van den Akker, S., Laetsch, D. R., Thorpe, P., Lilley, C. J., Danchin, E. G. J., Da Rocha, M., Rancurel, C., Holroyd, N. E., Cotton, J. A., Szitenberg, A., Grenier, E., Montarry, J., Mimee, B., Duceppe, M-O., Boyes, I., Marvin, J. M. C., Jones, L. M., Yusup, H. B., Lafond-Lapalme, J., Esquibet, M. & 14 othersSabeh, M., Rott, M., Overmars, H., Finkers-Tomczak, A., Smant, G., Koutsovoulos, G., Blok, V., Mantelin, S., Cock, P. J. A., Phillips, W., Henrissat, B., Urwin, P. E., Blaxter, M. & Jones, J. T., 10 Jun 2016, In : Genome Biology. 17, 1, p. 1-23 23 p., 124.

    Research output: Contribution to journalArticle

  4. 5-hydroxymethylcytosine marks promoters in colon that resist DNA hypermethylation in cancer

    Uribe-Lewis, S., Stark, R., Carroll, T., Dunning, M. J., Bachman, M., Ito, Y., Stojic, L., Halim, S., Vowler, S. L., Lynch, A. G., Delatte, B., de Bony, E. J., Colin, L., Defrance, M., Krueger, F., Silva, A. L., ten Hoopen, R., Ibrahim, A. E. K., Fuks, F. & Murrell, A., 1 Apr 2015, In : Genome Biology. 16, 15 p., 69.

    Research output: Contribution to journalArticle

  5. Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system

    Hsu, L-Y., Harris, S. R., Chlebowicz, M. A., Lindsay, J. A., Koh, T-H., Krishnan, P., Tan, T-Y., Hon, P-Y., Grubb, W. B., Bentley, S. D., Parkhill, J., Peacock, S. J. & Holden, M. T., 23 Apr 2015, In : Genome Biology. 16, 13 p., 81.

    Research output: Contribution to journalArticle

ID: 91754480