Skip to content

Research at St Andrews

PinR mediates the generation of reversible population diversity in Streptococcus zooepidemicus

Research output: Research - peer-reviewArticle

DOI

Open Access permissions

Open

Author(s)

Karen Frances Steward, Tihana Harrison, Carl Robinson, Josh Slater, Duncan J Maskell, Simon R Harris, Matthew T G Holden, Andrew S Waller

School/Research organisations

Abstract

Opportunistic pathogens must adapt to and survive in a wide range of complex ecosystems. Streptococcus zooepidemicus is an opportunistic pathogen of horses and many other animals, including man. The assembly of different surface architecture phenotypes from one genotype is likely to be crucial to the successful exploitation of such an opportunistic lifestyle. Construction of a series of mutants revealed that a serine recombinase, PinR, inverts 114 bp of the promoter of SZO_08560, which is bordered by GTAGACTTTA and TAAAGTCTAC inverted repeats. Inversion acts as a switch, controlling the transcription of this sortase-processed protein, which may enhance the attachment of S. zooepidemicus to equine trachea. The genome of a recently sequenced strain of S. zooepidemicus, strain 2329 (Sz2329), was found to contain a disruptive internal inversion of 7 kb of the FimIV pilus locus, which is bordered by TAGAAA and TTTCTA inverted repeats. This strain lacks pinR and we hypothesized that this inversion may have become irreversible following the loss of this recombinase. Active inversion of FimIV was detected in three strains of S. zooepidemicus: 1770 (Sz1770), B260863 (SzB260863) and H050840501 (SzH050840501), all of which encoded pinR. A deletion mutant of Sz1770 that lacked pinR was no longer capable of inverting its internal region of FimIV. Our data highlight redundancy in the PinR sequence recognition motif around a short TAGA consensus and suggest that PinR can reversibly influence the wider surface architecture of S. zooepidemicus, providing this organism with a bet-hedging solution to survival in fluctuating environments.

Close

Details

Original languageEnglish
Pages (from-to)1105-1112
JournalMicrobiology
Volume161
Issue number5
Early online date1 May 2015
DOIs
StatePublished - May 2015

    Research areas

  • Streptococcus zooepedemicus, PinR, Recombinase, Surface proteins

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing

    Bossé, J. T., Li, Y., Fernandez Crespo, R., Lacouture, S., Gottschalk, M., Sárközi, R., Fodor, L., Casas Amoribieta, M., Angen, Ø., Nedbalcova, K., Holden, M. T. G., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R. & on behalf of the BRaDP1T consortium 20 May 2018 In : Veterinary Microbiology. 220, p. 83-89 7 p.

    Research output: Research - peer-reviewArticle

  2. An outbreak of Streptococcus pyogenes in a mental health facility: advantage of well-timed whole-genome sequencing over emm typing

    Bergin, S. M. , Periaswamy, B. , Barkham, T. , Chua, H. C. , Mok, Y. M. , Fung, D. S. S. , Su, A. H. C. , Lee, Y. L. , Chua, M. L. I. , Ng, P. Y. , Soon, W. J. W. , Chu, C. W. , Tan, S. L. , Meehan, M. , Ang, B. S. P. , Leo, Y. S. , Holden, M. T. G. , De, P. , Hsu, L. Y. , Chen, S. L. & 2 others de Sessions, P. F. & Marimuthu, K. 9 May 2018 In : Infection Control and Hospital Epidemiology. First View, 9 p.

    Research output: Research - peer-reviewArticle

  3. The widespread use of topical antimicrobials enriches for resistance in Staphylococcus aureus isolated from Atopic Dermatitis patients

    Harkins, C. P., McAleer, M. A., Bennett, D., McHugh, M., Fleury, O. M., Pettigrew, K. A., Oravcová, K., Parkhill, J., Proby, C. M., Dawe, R. S., Geoghegan, J. A., Irvine, A. D. & Holden, M. T. G. 5 May 2018 (Accepted/In press) In : British Journal of Dermatology. In press

    Research output: Research - peer-reviewArticle

  4. Distinct evolutionary patterns of Neisseria meningitidis serogroup B disease outbreaks at two universities in the USA

    Hao, L., Holden, M. T. G., Wang, X., Andrew, L., Wellnitz, S., Hu, F., Whaley, M., Sammons, S., Knipe, K., Frace, M., McNamara, L. A., Liberator, P. & Anderson, A. S. Apr 2018 In : Microbial Genomics. 4, 4, 10 p., e000155

    Research output: Research - peer-reviewArticle

  5. Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis

    Bossé, J. T., Li, Y., Sárközi, R., Fodor, L., Lacouture, S., Gottschalk, M., Amoribieta, M. C., Angen, Ø., Nedbalcova, K., Holden, M. T. G., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R. & on behalf of the BRaDP1T consortium Apr 2018 In : Veterinary Microbiology. 217, p. 1-6

    Research output: Research - peer-reviewArticle

Related by journal

  1. Functional analysis of the EsaB component of the Staphylococcus aureus Type VII secretion system

    Casabona, M. G., Buchanan, G., Zoltner, M., Harkins, C. P., Holden, M. T. G. & Palmer, T. 1 Dec 2017 In : Microbiology. 163, 12, p. 1851-1863 13 p.

    Research output: Research - peer-reviewArticle

  2. Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390

    Casabona, M. G., Kneuper, H., Alferes de Lima, D., Harkins, C. P., Zoltner, M., Hjerde, E., Holden, M. T. G. & Palmer, T. 1 Dec 2017 In : Microbiology. 163, 12, p. 1839-1850

    Research output: Research - peer-reviewArticle

  3. High-affinity nitrate/nitrite transporters NrtA and NrtB of Aspergillus nidulans exhibit high specificity and different inhibitor sensitivity

    Akhtar, N., Karabika, E., Kinghorn, J. R., Glass, A. D. M., Unkles, S. E. & Rouch, D. A. 1 Jul 2015 In : Microbiology. 161, 7, p. 1435-1446

    Research output: Research - peer-reviewArticle

Related by journal

  1. Microbiology (Journal)

    Holden, M. (Editor)
    2013

    Activity: Publication peer-review and editorial workEditor of research journal

ID: 174263266