Skip to content

Research at St Andrews

Plasmodium vivax merozoite surface protein 1 paralog as a mediator of parasite adherence to reticulocytes

Research output: Contribution to journalArticlepeer-review


Open Access permissions



Jin-Hee Han, Jee-Sun Cho, Yang Cheng, Fauzi Muh, Won Gi Yoo, Bruce Russell, Francois Nosten, Sunghun Na, Kwon-Soo Ha, Won Sun Park, Seok-Ho Hong, Eun-Taek Han

School/Research organisations


Plasmodium vivax parasites preferentially invade reticulocytes in human beings. P. vivax merozoite surface protein 1 (PvMSP1) and PvMSP1 paralog (PvMSP1P) may have important functions in reticulocyte adherence during invasion. These proteins share similar structures, including the presence of two epidermal growth factor (EGF)-like and glycosylphosphatidylinositol (GPI)-anchored domains at the C terminus. However, there have been no reports concerning the functional activity of PvMSP1P in reticulocyte adherence during P. vivax invasion. In this study, the ability of PvMSP1P-19 to bind to reticulocytes and normocytes was analyzed. The reticulocyte binding activity of PvMSP1P-19 was 4.0-fold higher than its normocyte binding activity. The binding of PvMSP1 P-19 to reticulocytes and normocytes was inhibited in a dose-dependent manner by antibodies from immunized rabbits and by antibodies from vivax parasite-infected patients. Consistently, antibodies against PvMSP1 P inhibited parasite invasion during short-term in vitro cultivation. Similar to the case for PvDBPII binding activity, PvMSP1P-19 binding activity was reduced in chymotrypsin-treated reticulocytes. However, no significant difference between the binding of PvMSP1P-19 to Duffy-positive and Duffy-negative erythrocytes was found. The minimal binding motif of PvMSP1P-19 was characterized using synthetic peptides. The results showed that the residues at amino acid positions 1791 to 1808 may have an important function in mediating merozoite adherence to reticulocytes. The positively charged residues within the EGF-like domain were shown to constitute a key binding motif. This work presents strong evidence supporting the role of PvMSP1P in host target cell selection and invasion of Duffy-independent pathway in P. vivax. Moreover, PvMSP1P-19-specific antibodies may confer protection against P. vivax reinvasion.



Original languageEnglish
Article numberARTN e00239-18
Number of pages13
JournalInfection and Immunity
Issue number9
Early online date22 Aug 2018
Publication statusPublished - Sep 2018

    Research areas

  • Plasmodium vivax, Merozoite surface, Duffy independent, Merozoite surface protein 1, Merozoite surface protein 1 paralog, Invasion inhibition, Reticulocyte

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Cross-species reactivity of antibodies against Plasmodium vivax blood-stage antigens to Plasmodium knowlesi

    Fauzi, M., Kim, N., Nyunt, M. H., Firdaus, E. R., Han, J-H., Hoque, M. R., Lee, S-K., Park, J-H., Moon, R. W., Lau, Y. L., Kaneko, O. & Han, E-T., 19 Jun 2020, In: PLoS Neglected Tropical Diseases. 14, 6, 21 p., e0008323.

    Research output: Contribution to journalArticlepeer-review

  2. The acquisition of long-lived memory B cell responses to merozoite surface protein-8 in individuals with Plasmodium vivax infection

    Kochayoo, P., Kittisenachai, N., Changrob, S., Wangriatisak, K., Muh, F., Chootong, P. & Han, E-T., 31 May 2019, In: Malaria Journal. 18, 10 p., 188.

    Research output: Contribution to journalArticlepeer-review

  3. Inhibition of parasite invasion by monoclonal antibody against epidermal growth factor-like domain of Plasmodium vivax merozoite surface protein 1 paralog

    Han, J-H., Cheng, Y., Muh, F., Ahmed, M. A., Cho, J-S., Nyunt, M. H., Jeon, H-Y., Ha, K-S., Na, S., Park, W. S., Hong, S-H., Shin, H-J., Russell, B. & Han, E-T., 7 Mar 2019, In: Scientific Reports. 9, 11 p., 3906.

    Research output: Contribution to journalArticlepeer-review

  4. Diversity pattern of Duffy binding protein sequence among Duffy-negatives and Duffy-positives in Sudan

    Hoque, M. R., Elfaki, M. M. A., Ahmed, M. A., Lee, S-K., Muh, F., Albsheer, M. M. A., Hamid, M. M. A. & Han, E-T., 17 Aug 2018, In: Malaria Journal. 17, 10 p., 297.

    Research output: Contribution to journalArticlepeer-review

  5. In vitro invasion inhibition assay using antibodies against Plasmodium knowlesi Duffy binding protein alpha and apical membrane antigen protein 1 in human erythrocyte-adapted P. knowlesi A1-H.1 strain

    Muh, F., Lee, S-K., Hoque, M. R., Han, J-H., Park, J-H., Firdaus, E. R., Moon, R. W., Lau, Y. L. & Han, E-T., 27 Jul 2018, In: Malaria Journal. 17, 11 p., 272.

    Research output: Contribution to journalArticlepeer-review

Related by journal

  1. Colony morphology variation of Burkholderia pseudomallei is associated with antigenic variation and O-polysaccharide modifications

    Wikraiphat, C., Saiprom, N., Tandhavanant, S., Heiss, C., Azadi, P., Wongsuvan, G., Tuanyok, A., Holden, M., Burtnick, M. N., Brett, P. J., Peacock, S. J. & Chantratita, N., May 2015, In: Infection and Immunity. 83, 5, p. 2127-2138

    Research output: Contribution to journalArticlepeer-review

  2. Identification of Three Novel Superantigen-Encoding Genes in Streptococcus equi subsp zooepidemicus, szeF, szeN, and szeP

    Paillot, R., Darby, A. C., Robinson, C., Wright, N. L., Steward, K. F., Anderson, E., Webb, K., Holden, M. T. G., Efstratiou, A., Broughton, K., Jolley, K. A., Priestnall, S. L., Campi, M. C. M., Hughes, M. A., Radford, A., Erles, K. & Waller, A. S., Nov 2010, In: Infection and Immunity. 78, 11, p. 4817-4827 11 p.

    Research output: Contribution to journalArticlepeer-review

  3. Allelic variation in Streptococcus pneumoniae autolysin (N- Acetyl muramoyl-L-alanine amidase)

    Gillespie, S. H., McHugh, T. D., Ayres, H., Dickens, A., Efstratiou, A. & Whiting, G. C., 1 Oct 1997, In: Infection and Immunity. 65, 9, p. 3936-3938 3 p.

    Research output: Contribution to journalArticlepeer-review

  4. Species of alpha-hemolytic streptococci possessing a C-polysaccharide phosphorylcholine-containing antigen

    Gillespie, S. H., McWhinney, P. H. M., Patel, S., Raynes, J. G., McAdam, K. P. W. J., Whiley, R. A. & Hardie, J. M., 6 Jul 1993, In: Infection and Immunity. 61, 7, p. 3076-3077 2 p.

    Research output: Contribution to journalComment/debatepeer-review

ID: 259675068