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Plasmodium vivax merozoite surface protein 1 paralog as a mediator of parasite adherence to reticulocytes

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Jin-Hee Han, Jee-Sun Cho, Yang Cheng, Fauzi Muh, Won Gi Yoo, Bruce Russell, Francois Nosten, Sunghun Na, Kwon-Soo Ha, Won Sun Park, Seok-Ho Hong, Eun-Taek Han

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Abstract

Plasmodium vivax parasites preferentially invade reticulocytes in human beings. P. vivax merozoite surface protein 1 (PvMSP1) and PvMSP1 paralog (PvMSP1P) may have important functions in reticulocyte adherence during invasion. These proteins share similar structures, including the presence of two epidermal growth factor (EGF)-like and glycosylphosphatidylinositol (GPI)-anchored domains at the C terminus. However, there have been no reports concerning the functional activity of PvMSP1P in reticulocyte adherence during P. vivax invasion. In this study, the ability of PvMSP1P-19 to bind to reticulocytes and normocytes was analyzed. The reticulocyte binding activity of PvMSP1P-19 was 4.0-fold higher than its normocyte binding activity. The binding of PvMSP1 P-19 to reticulocytes and normocytes was inhibited in a dose-dependent manner by antibodies from immunized rabbits and by antibodies from vivax parasite-infected patients. Consistently, antibodies against PvMSP1 P inhibited parasite invasion during short-term in vitro cultivation. Similar to the case for PvDBPII binding activity, PvMSP1P-19 binding activity was reduced in chymotrypsin-treated reticulocytes. However, no significant difference between the binding of PvMSP1P-19 to Duffy-positive and Duffy-negative erythrocytes was found. The minimal binding motif of PvMSP1P-19 was characterized using synthetic peptides. The results showed that the residues at amino acid positions 1791 to 1808 may have an important function in mediating merozoite adherence to reticulocytes. The positively charged residues within the EGF-like domain were shown to constitute a key binding motif. This work presents strong evidence supporting the role of PvMSP1P in host target cell selection and invasion of Duffy-independent pathway in P. vivax. Moreover, PvMSP1P-19-specific antibodies may confer protection against P. vivax reinvasion.

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Original languageEnglish
Article numberARTN e00239-18
Number of pages13
JournalInfection and Immunity
Volume86
Issue number9
Early online date22 Aug 2018
DOIs
Publication statusPublished - Sep 2018

    Research areas

  • Plasmodium vivax, Merozoite surface, Duffy independent, Merozoite surface protein 1, Merozoite surface protein 1 paralog, Invasion inhibition, Reticulocyte

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