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Potato mop-top virus co-opts the stress sensor HIPP26 for long-distance movement

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Author(s)

Graham H. Cowan, Alison G. Roberts, Susan Jones, Pankaj Kumar, Pruthvi B. Kalyandurg, Jose F. Gil, Eugene I. Savenkov, Piers A. Hemsley, Lesley Torrance

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Abstract

Virus movement proteins facilitate virus entry into the vascular system to initiate systemic infection. The potato mop-top virus (PMTV) movement protein, TGB1, is involved in long-distance movement of both viral ribonucleoprotein complexes and virions. Here, our analysis of TGB1 interactions with host Nicotiana benthamiana proteins revealed an interaction with a member of the heavy metal-associated isoprenylated plant protein family, HIPP26, which acts as a plasma membrane-to-nucleus signal during abiotic stress. We found that knockdown of NbHIPP26 expression inhibited virus long-distance movement but did not affect cell-to-cell movement. Drought and PMTV infection up-regulated NbHIPP26 gene expression, and PMTV infection protected plants from drought. In addition, NbHIPP26 promoter-reporter fusions revealed vascular tissue-specific expression. Mutational and biochemical analyses indicated that NbHIPP26 subcellular localization at the plasma membrane and plasmodesmata was mediated by lipidation (S-acylation and prenylation), as nonlipidated NbHIPP26 was predominantly in the nucleus. Notably, coexpression of NbHIPP26 with TGB1 resulted in a similar nuclear accumulation of NbHIPP26. TGB1 interacted with the carboxyl-terminal CVVM (prenyl) domain of NbHIPP26, and bimolecular fluorescence complementation revealed that the TGB1-HIPP26 complex localized to microtubules and accumulated in the nucleolus, with little signal at the plasma membrane or plasmodesmata. These data support a mechanism where interaction with TGB1 negates or reverses NbHIPP26 lipidation, thus releasing membrane-associated NbHIPP26 and redirecting it via microtubules to the nucleus, thereby activating the drought stress response and facilitating virus long-distance movement.

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Original languageEnglish
Pages (from-to)2052-2070
Number of pages19
JournalPlant Physiology
Volume176
Issue number3
DOIs
Publication statusPublished - 6 Mar 2018

    Research areas

  • NAC transcription factors, Nicotiana-benthamiana, Nucleolar localization, Plant transformation, Functional-analysis, Brassica-napus, Small RNAs, Protein, Phloem, Arabidopsis

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