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Profiling of the embryonic Atlantic halibut (Hippoglossus hippoglossus L.) transcriptome reveals maternal transcripts as potential markers of embryo quality

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Profiling of the embryonic Atlantic halibut (Hippoglossus hippoglossus L.) transcriptome reveals maternal transcripts as potential markers of embryo quality. / Mommens, Maren; Fernandes, Jorge M. O.; Tollefsen, Knut Erik; Johnston, Ian A.; Babiak, Igor.

In: BMC Genomics, Vol. 15, 829, 30.09.2014.

Research output: Contribution to journalArticle

Harvard

Mommens, M, Fernandes, JMO, Tollefsen, KE, Johnston, IA & Babiak, I 2014, 'Profiling of the embryonic Atlantic halibut (Hippoglossus hippoglossus L.) transcriptome reveals maternal transcripts as potential markers of embryo quality', BMC Genomics, vol. 15, 829. https://doi.org/10.1186/1471-2164-15-829

APA

Mommens, M., Fernandes, J. M. O., Tollefsen, K. E., Johnston, I. A., & Babiak, I. (2014). Profiling of the embryonic Atlantic halibut (Hippoglossus hippoglossus L.) transcriptome reveals maternal transcripts as potential markers of embryo quality. BMC Genomics, 15, [829]. https://doi.org/10.1186/1471-2164-15-829

Vancouver

Mommens M, Fernandes JMO, Tollefsen KE, Johnston IA, Babiak I. Profiling of the embryonic Atlantic halibut (Hippoglossus hippoglossus L.) transcriptome reveals maternal transcripts as potential markers of embryo quality. BMC Genomics. 2014 Sep 30;15. 829. https://doi.org/10.1186/1471-2164-15-829

Author

Mommens, Maren ; Fernandes, Jorge M. O. ; Tollefsen, Knut Erik ; Johnston, Ian A. ; Babiak, Igor. / Profiling of the embryonic Atlantic halibut (Hippoglossus hippoglossus L.) transcriptome reveals maternal transcripts as potential markers of embryo quality. In: BMC Genomics. 2014 ; Vol. 15.

Bibtex - Download

@article{93bccbb11561456298f44b48351f614d,
title = "Profiling of the embryonic Atlantic halibut (Hippoglossus hippoglossus L.) transcriptome reveals maternal transcripts as potential markers of embryo quality",
abstract = "Background: Commercial Atlantic halibut (Hippoglossus hippoglossus) farming is restricted by variable oocyte quality, slow growth, and early maturation of male fish. Maternally transferred components regulate early developmental processes; therefore, they have an effect on the future viability of the embryo. Using a newly developed Agilent 10 k custom-made oligonucleotide array, we profiled components of the transcriptome involved in immune defence as well as germline and muscle development during early developmental stages: 8-cell embryos (8CS), germ ring stage (GR), 10-somite stage (10SS), and hatched embryos (HT). In addition, we identified differentially expressed transcripts in low (= 86 +/- 3 degrees{\%} hatching) quality eggs at 8CS to identify potential maternal markers for embryo quality.Results: Out of 2066 differentially expressed transcripts, 160 were identified as maternal transcripts being specifically expressed at 8CS only. Twenty transcripts were differentially expressed in 8-cell embryos between low and high quality egg groups. Several immune-related transcripts were identified as promising molecular markers of hatching success including interferon regulatory factor 7 and mhc class 2A chain. Differential expression was positively validated with quantitative real-time PCR.Conclusions: We have demonstrated maternal transfer of innate and adaptive immune system transcripts into Atlantic halibut embryos and their relation with future embryo developmental potential. We identified several transcripts as potential molecular markers of embryo quality. The developed microarray represents a useful resource for improving the commercial production of Atlantic halibut.",
keywords = "Atlantic halibut, Egg quality, Embryonic development, Maternal transcripts, Microarray, Transcriptome, Trout oncorhynchus-mykiss, Messenger-RNA stockpile, Primordial germ-cells, Developmental competence, Gene-expression, Flesh quality, Dead-end, Zebrafish, Migration",
author = "Maren Mommens and Fernandes, {Jorge M. O.} and Tollefsen, {Knut Erik} and Johnston, {Ian A.} and Igor Babiak",
note = "This study was funded by the Research Council of Norway (Project nr. 182653/V10) and the NIVA SIS project “MolPOP”.",
year = "2014",
month = "9",
day = "30",
doi = "10.1186/1471-2164-15-829",
language = "English",
volume = "15",
journal = "BMC Genomics",
issn = "1471-2164",
publisher = "BioMed Central",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Profiling of the embryonic Atlantic halibut (Hippoglossus hippoglossus L.) transcriptome reveals maternal transcripts as potential markers of embryo quality

AU - Mommens, Maren

AU - Fernandes, Jorge M. O.

AU - Tollefsen, Knut Erik

AU - Johnston, Ian A.

AU - Babiak, Igor

N1 - This study was funded by the Research Council of Norway (Project nr. 182653/V10) and the NIVA SIS project “MolPOP”.

PY - 2014/9/30

Y1 - 2014/9/30

N2 - Background: Commercial Atlantic halibut (Hippoglossus hippoglossus) farming is restricted by variable oocyte quality, slow growth, and early maturation of male fish. Maternally transferred components regulate early developmental processes; therefore, they have an effect on the future viability of the embryo. Using a newly developed Agilent 10 k custom-made oligonucleotide array, we profiled components of the transcriptome involved in immune defence as well as germline and muscle development during early developmental stages: 8-cell embryos (8CS), germ ring stage (GR), 10-somite stage (10SS), and hatched embryos (HT). In addition, we identified differentially expressed transcripts in low (= 86 +/- 3 degrees% hatching) quality eggs at 8CS to identify potential maternal markers for embryo quality.Results: Out of 2066 differentially expressed transcripts, 160 were identified as maternal transcripts being specifically expressed at 8CS only. Twenty transcripts were differentially expressed in 8-cell embryos between low and high quality egg groups. Several immune-related transcripts were identified as promising molecular markers of hatching success including interferon regulatory factor 7 and mhc class 2A chain. Differential expression was positively validated with quantitative real-time PCR.Conclusions: We have demonstrated maternal transfer of innate and adaptive immune system transcripts into Atlantic halibut embryos and their relation with future embryo developmental potential. We identified several transcripts as potential molecular markers of embryo quality. The developed microarray represents a useful resource for improving the commercial production of Atlantic halibut.

AB - Background: Commercial Atlantic halibut (Hippoglossus hippoglossus) farming is restricted by variable oocyte quality, slow growth, and early maturation of male fish. Maternally transferred components regulate early developmental processes; therefore, they have an effect on the future viability of the embryo. Using a newly developed Agilent 10 k custom-made oligonucleotide array, we profiled components of the transcriptome involved in immune defence as well as germline and muscle development during early developmental stages: 8-cell embryos (8CS), germ ring stage (GR), 10-somite stage (10SS), and hatched embryos (HT). In addition, we identified differentially expressed transcripts in low (= 86 +/- 3 degrees% hatching) quality eggs at 8CS to identify potential maternal markers for embryo quality.Results: Out of 2066 differentially expressed transcripts, 160 were identified as maternal transcripts being specifically expressed at 8CS only. Twenty transcripts were differentially expressed in 8-cell embryos between low and high quality egg groups. Several immune-related transcripts were identified as promising molecular markers of hatching success including interferon regulatory factor 7 and mhc class 2A chain. Differential expression was positively validated with quantitative real-time PCR.Conclusions: We have demonstrated maternal transfer of innate and adaptive immune system transcripts into Atlantic halibut embryos and their relation with future embryo developmental potential. We identified several transcripts as potential molecular markers of embryo quality. The developed microarray represents a useful resource for improving the commercial production of Atlantic halibut.

KW - Atlantic halibut

KW - Egg quality

KW - Embryonic development

KW - Maternal transcripts

KW - Microarray

KW - Transcriptome

KW - Trout oncorhynchus-mykiss

KW - Messenger-RNA stockpile

KW - Primordial germ-cells

KW - Developmental competence

KW - Gene-expression

KW - Flesh quality

KW - Dead-end

KW - Zebrafish

KW - Migration

UR - http://www.biomedcentral.com/1471-2164/15/829/additional

U2 - 10.1186/1471-2164-15-829

DO - 10.1186/1471-2164-15-829

M3 - Article

VL - 15

JO - BMC Genomics

JF - BMC Genomics

SN - 1471-2164

M1 - 829

ER -

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