Skip to content

Research at St Andrews

Raman spectroscopy investigation of biochemical changes in tumor spheroids with aging and after treatment with staurosporine

Research output: Contribution to journalArticle

DOI

Open Access Status

  • Embargoed (until 9/01/20)

Author(s)

Lauren E. Jamieson, David J. Harrison, Colin J. Campbell

School/Research organisations

Abstract

There has been increasing use of in vitro cell culture models that more realistically replicate the three dimensional (3D) environment found in vivo. Multicellular tumour spheroids (MTS) using cell lines or patient-derived organoids have become an important in vitro drug development tool, where cells are grown in a 3D 'sphere' that exhibits many of the characteristics found in vivo. Significantly, MTS develop gradients in nutrients and oxygen, commonly found in tumours, that contribute to therapy resistance. While MTS show promise as a more realistic in vitro culture model, there is a massive need to improve imaging technologies for assessing biochemical characteristics and drug response in such models to maximize their translation into useful applications such as high throughput screening (HTS). In this study we investigate the potential for Raman spectroscopy to unveil biochemical information in MTS and have investigated how spheroid age influences drug response, shedding light on increased therapy resistance in developing tumours. The wealth of molecular level information delivered by Raman spectroscopy in a noninvasive manner, could aid translation of these 3D models into HTS applications.
Close

Details

Original languageEnglish
Article numbere201800201
JournalJournal of Biophotonics
Volume12
Issue number5
Early online date9 Jan 2019
DOIs
Publication statusPublished - May 2019

    Research areas

  • Multicellular tumour spheroids, Raman, Cancer, Drug screening

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. The landscape of genomic copy number alterations in colorectal cancer and their consequences on gene expression levels and disease outcome

    Ried, T., Meijer, G. A., Harrison, D. J., Grech, G., Franch-Expósito, S., Briffa, R., Carvalho, B. & Camps, J., Oct 2019, In : Molecular Aspects of Medicine. 69, p. 48-61 14 p.

    Research output: Contribution to journalArticle

  2. Genome-scale CRISPR/Cas9 screen determines factors modulating sensitivity to ProTide NUC-1031

    Sarr, A., Bré, J., Um, I. H., Chan, T. H., Mullen, P., Harrison, D. J. & Reynolds, P. A., 21 May 2019, In : Scientific Reports. 9, 13 p., 7643.

    Research output: Contribution to journalArticle

  3. Automated tumour budding quantification by machine learning augments TNM staging in muscle-invasive bladder cancer prognosis

    Brieu, N., Gavriel, C., Nearchou, I. P., Harrison, D. J., Schmidt, G. & Caie, P. D., 26 Mar 2019, In : Scientific Reports. 9, 5174.

    Research output: Contribution to journalArticle

Related by journal

  1. Depth-resolved multimodal imaging: wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography

    Chen, M., Mas, J., Forbes, L. H., Andrews, M. R. & Dholakia, K., Jan 2018, In : Journal of Biophotonics. 11, 1, 7 p., e201700129.

    Research output: Contribution to journalArticle

  2. Towards automated cancer screening: label-free classification of fixed cell samples using wavelength modulated Raman spectroscopy

    Woolford, L., Chen, M., Dholakia, K. & Herrington, C. S., Apr 2018, In : Journal of Biophotonics. 11, 4, e201700244.

    Research output: Contribution to journalArticle

  3. Cell perforation mediated by plasmonic bubbles generated by a single near infrared femtosecond laser pulse

    Boutopoulos, C., Bergeron, E. & Meunier, M., 2015, In : Journal of Biophotonics.

    Research output: Contribution to journalArticle

  4. Volumetric hand-held optoacoustic angiography as a tool for real-time screening of dense breast

    Deán-Ben, X. L., Fehm, T. F., Gostic, M. & Razansky, D., 2015, In : Journal of Biophotonics.

    Research output: Contribution to journalArticle

ID: 256025534

Top