Skip to content

Research at St Andrews

Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes

Research output: Contribution to journalArticle

Author(s)

Christopher Hauton, JA Hammond, Valerie Jane Smith

School/Research organisations

Abstract

This paper presents data from an investigation of the mode of action of five different crustacean immunostimulants presented to European lobster (Homarus gammarus) granulocytes cultured in vitro. The experiments were designed to test whether or not the immunostimulants could cause the short-term up-regulation of genes coding for immune proteins without causing the cells to degranulate. Quantitative measurements of mRNA transcript abundance were made using real-time PCR and it was first necessary to isolate the complete gene sequences coding for the proteins prophenoloxidase (proPO), beta-1,3-glucan binding protein (betaGBP) and beta-actin (beta-act) in the lobster. These sequences were used to design TaqMan(TM) primer and fluorescent probe sets. The presented data indicated that the majority of the tested immunostimulants did not induce the up-regulation of immune-related gene expression in the granulocytes in isolation. Alternative modes of action, including the in vivo up-regulation of gene expression in haemopoetic tissues, are discussed. (C) 2004 Elsevier Ltd. All rights reserved.

Close

Details

Original languageEnglish
Pages (from-to)33-42
Number of pages10
JournalDevelopmental and Comparative Immunology
Volume29
Issue number1
DOIs
Publication statusPublished - Jan 2005

    Research areas

  • real-time PCR, immunostimulants, prophenoloxidase, beta-1,3-glucan binding protein, lobster, Homarus gammarus, granulocytes, PROPHENOLOXIDASE-ACTIVATING SYSTEM, CRAYFISH PACIFASTACUS-LENIUSCULUS, BETA-1,3-GLUCAN BINDING-PROTEIN, FRESH-WATER CRAYFISH, MESSENGER-RNA LEVELS, BLACK TIGER SHRIMP, PENAEUS-MONODON, RT-PCR, LIPOPOLYSACCHARIDE, AQUACULTURE

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by journal

  1. Developmental and Comparative Immunology (Journal)

    Val Smith (Member of editorial board)
    20122018

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  2. Developmental and Comparative Immunology (Journal)

    Val Smith (Member of editorial board)
    20082015

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. Unusual tissue distribution of carcinin, an antibacterial crustin, in the crab, Carcinus maenas, reveals its multi-functionality

    Suleiman, S., Smith, V. J. & Dyrynda, E. A., Nov 2017, In : Developmental and Comparative Immunology. 76, p. 274-284

    Research output: Contribution to journalArticle

  2. Diverse immune functions of hemocyanins

    Coates, C. J. & Nairn, J., Jul 2014, In : Developmental and Comparative Immunology. 45, 1, p. 43-55

    Research output: Contribution to journalArticle

  3. Isolation and in vitro characterisation of prohaemocytes from the spider crab, Hyas araneus (L.)

    Roulston, C. & Smith, V. J., May 2011, In : Developmental and Comparative Immunology. 35, 5, p. 537-544

    Research output: Contribution to journalArticle

  4. Possible role of phosphatidylserine-hemocyanin interaction in the innate immune response of Limulus polyphemus

    Coates, C. J., Kelly, S. M. & Nairn, J., Feb 2011, In : Developmental and Comparative Immunology. 35, 2, p. 155-163 9 p.

    Research output: Contribution to journalArticle

  5. Expression of antimicrobial peptides from Hyas araneus hemocytes following bacterial challenge in vitro

    Sperstad, S., Smith, V. J. & Stensvag, K., 2010, In : Developmental and Comparative Immunology. 34, p. 618–624

    Research output: Contribution to journalArticle

ID: 300004