Skip to content

Research at St Andrews

Reduced plasma magnesium levels in type-1 diabetes associate with prothrombotic changes in fibrin clotting and fibrinolysis

Research output: Contribution to journalArticle

Abstract

Individuals with type-1 diabetes mellitus (T1DM) have a higher risk of thrombosis and low plasma magnesium concentrations. As magnesium is a known regulator of fibrin network formation, we investigated potential associations between fibrin clot properties and plasma magnesium concentrations in 45 individuals with T1DM and 47 age- and sex-matched controls without diabetes. Fibrin clot characteristics were assessed using a validated turbidimetric assay and associations with plasma magnesium concentration were examined. Plasma concentrations of fibrinogen, plasminogen activator inhibitor-1 (PAI-1), and lipids were measured and fibrin fiber diameters assessed using scanning electron microscopy. Fibrin clot maximum absorbance was unchanged in subjects with T1DM compared with controls, while lysis time was prolonged (p = 0.0273). No differences in fibrin fiber diameters or in lipid profile were observed between T1DM and controls. PAI-1 concentration was lower in the T1DM group compared with the controls (p = 0.0232) and positively correlated with lysis time (p = 0.0023). Plasma magnesium concentration was lower in the T1DM group compared with controls (p < 0.0001). Magnesium concentration negatively correlated with clot maximum absorbance (p = 0.0215) and lysis time (p = 0.0464). A turbidimetric fibrin clot lysis assay performed in a purified system that included PAI-1 and 0 to 3.2 mM Mg2+ showed a shortening of lysis time with increasing Mg2+ concentrations (p = 0.0004). Our findings reveal that plasma magnesium concentration is associated with changes in fibrin clot and lysis parameters.
Close

Details

Original languageEnglish
Number of pages10
JournalThrombosis and Haemostasis
VolumeeFirst
Early online date3 Jan 2020
DOIs
Publication statusE-pub ahead of print - 3 Jan 2020

    Research areas

  • Clot lysis, Coagulation, Metal homeostasis, Plasminogen activator inhibitor-1, Thrombosis

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Plasma non-esterified fatty acids contribute to increased coagulability in type-2 diabetes through altered plasma zinc speciation

    Sobczak, A. I. S., Katundu, K. G. H., Phoenix, F. A., Khazaipoul, S., Yu, R., Lampiao, F., Stefanowicz, F., Blindauer, C. A., Pitt, S. J., Smith, T. K., Ajjan, R. A. & Stewart, A. J., 28 Aug 2019, In : biorxiv. 38 p.

    Research output: Contribution to journalArticle

  2. Total plasma magnesium, zinc, copper and selenium concentrations in type-I and type-II diabetes

    Sobczak, A. I. S., Stefanowicz, F., Pitt, S. J., Ajjan, R. A. & Stewart, A. J., Feb 2019, In : BioMetals. 32, 1, p. 123-138 16 p.

    Research output: Contribution to journalArticle

  3. Influence of zinc on glycosaminoglycan neutralisation during coagulation

    Sobczak, A. I. S., Pitt, S. J. & Stewart, A. J., Sep 2018, In : Metallomics. 10, 9, p. 1180-1190 11 p.

    Research output: Contribution to journalReview article

  4. Glycosaminoglycan neutralization in coagulation control

    Sobczak, A. I. S., Pitt, S. J. & Stewart, A. J., Jun 2018, In : Arteriosclerosis, Thrombosis, and Vascular Biology. 38, 6, p. 1258-1270 14 p.

    Research output: Contribution to journalReview article

  5. Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high fat diet

    Jovanovic, A., Obradovic, M., Sudar-Milovanovic, E., Stewart, A. J., Pitt, S. J., Alvantic, D., Aleksic, E. & Isenovic, E. R., Dec 2017, In : Molecular and Cellular Biochemistry. 436, 1-2, p. 49-58 10 p.

    Research output: Contribution to journalArticle

ID: 263036757

Top