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Regulation of expression of two aquaporin homologs in the intestine of the European eel: effects of seawater acclimation and cortisol treatment.

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Regulation of expression of two aquaporin homologs in the intestine of the European eel: effects of seawater acclimation and cortisol treatment. / Martinez, Anne-Sophie; Cutler, Christopher Paul; Wilson, G D; Phillips, C; Hazon, Neil; Cramb, Gordon.

In: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, Vol. 288, No. 6, 06.2005, p. R1733-R1743.

Research output: Contribution to journalArticle

Harvard

Martinez, A-S, Cutler, CP, Wilson, GD, Phillips, C, Hazon, N & Cramb, G 2005, 'Regulation of expression of two aquaporin homologs in the intestine of the European eel: effects of seawater acclimation and cortisol treatment.' American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, vol. 288, no. 6, pp. R1733-R1743. https://doi.org/10.1152/ajpregu.00747.2004

APA

Martinez, A-S., Cutler, C. P., Wilson, G. D., Phillips, C., Hazon, N., & Cramb, G. (2005). Regulation of expression of two aquaporin homologs in the intestine of the European eel: effects of seawater acclimation and cortisol treatment. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 288(6), R1733-R1743. https://doi.org/10.1152/ajpregu.00747.2004

Vancouver

Martinez A-S, Cutler CP, Wilson GD, Phillips C, Hazon N, Cramb G. Regulation of expression of two aquaporin homologs in the intestine of the European eel: effects of seawater acclimation and cortisol treatment. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology. 2005 Jun;288(6):R1733-R1743. https://doi.org/10.1152/ajpregu.00747.2004

Author

Martinez, Anne-Sophie ; Cutler, Christopher Paul ; Wilson, G D ; Phillips, C ; Hazon, Neil ; Cramb, Gordon. / Regulation of expression of two aquaporin homologs in the intestine of the European eel: effects of seawater acclimation and cortisol treatment. In: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology. 2005 ; Vol. 288, No. 6. pp. R1733-R1743.

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@article{73b1060903e94a32ab429bde74c43dad,
title = "Regulation of expression of two aquaporin homologs in the intestine of the European eel: effects of seawater acclimation and cortisol treatment.",
abstract = "Complementary DNAs encoding homologs of the mammalian aqua-glyceroporins (termed AQPe) and aquaporin-1 isoforms (termed AQP1) were isolated from the European eel. The AQP amino acid sequences share 35 - 54{\%} identity with other known human AQPs. Although AQPe mRNA expression was approximately equivalent along the entire length of the gut, AQP1 expression was the highest in the posterior/rectal segment. Seawater ( SW) acclimation increased AQP1 mRNA abundance by 5- and 17-fold in the anterior, 14- and 23-fold in the mid-, and 9- and 17-fold in the posterior/ rectal gut regions of yellow and silver eels, respectively. SW acclimation had an effect on AQPe mRNA expression only in the midintestine of silver eels, where a small but significant 1.7-fold increase in abundance was measured. Western blots using an eel AQP1-specific antibody identified the presence of a major immunoreactive 28-kDa protein, primarily within the posterior/ rectal segment. A 3-wk SW transfer induced an increase in AQP1 protein abundance in all intestinal segments, with the posterior/ rectal region still expressing protein levels ∼ 40- and 8-fold higher than the anterior and midsegments, respectively. Strong AQP1 immunofluorescence was detected within the vascular endothelium in both freshwater (FW)- and SW-acclimated eels and in the epithelial apical brush border in the posterior/ rectal gut regions of SW-acclimated eels. Cortisol infusion into FW eels had no effect on intestinal AQPe mRNA expression but induced increases in AQP1 mRNA and protein levels. These results provide evidence for the presence of a SW-induced and steroid-regulated AQP water channel pathway within the intestine of the European eel.",
keywords = "Anguilla anguilla, gastrointestinal tract, AQP1, AQPe, corticosteroid, ANGUILLA-ANGUILLA, WATER TRANSPORT, GASTROINTESTINAL-TRACT, GLANDULAR TISSUES, GENE-EXPRESSION, COMPLEX TISSUES, MESSENGER-RNA, JAPONICA, ABSORPTION, CHANNELS",
author = "Anne-Sophie Martinez and Cutler, {Christopher Paul} and Wilson, {G D} and C Phillips and Neil Hazon and Gordon Cramb",
year = "2005",
month = "6",
doi = "10.1152/ajpregu.00747.2004",
language = "English",
volume = "288",
pages = "R1733--R1743",
journal = "American Journal of Physiology. Regulatory, Integrative and Comparative Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "6",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Regulation of expression of two aquaporin homologs in the intestine of the European eel: effects of seawater acclimation and cortisol treatment.

AU - Martinez, Anne-Sophie

AU - Cutler, Christopher Paul

AU - Wilson, G D

AU - Phillips, C

AU - Hazon, Neil

AU - Cramb, Gordon

PY - 2005/6

Y1 - 2005/6

N2 - Complementary DNAs encoding homologs of the mammalian aqua-glyceroporins (termed AQPe) and aquaporin-1 isoforms (termed AQP1) were isolated from the European eel. The AQP amino acid sequences share 35 - 54% identity with other known human AQPs. Although AQPe mRNA expression was approximately equivalent along the entire length of the gut, AQP1 expression was the highest in the posterior/rectal segment. Seawater ( SW) acclimation increased AQP1 mRNA abundance by 5- and 17-fold in the anterior, 14- and 23-fold in the mid-, and 9- and 17-fold in the posterior/ rectal gut regions of yellow and silver eels, respectively. SW acclimation had an effect on AQPe mRNA expression only in the midintestine of silver eels, where a small but significant 1.7-fold increase in abundance was measured. Western blots using an eel AQP1-specific antibody identified the presence of a major immunoreactive 28-kDa protein, primarily within the posterior/ rectal segment. A 3-wk SW transfer induced an increase in AQP1 protein abundance in all intestinal segments, with the posterior/ rectal region still expressing protein levels ∼ 40- and 8-fold higher than the anterior and midsegments, respectively. Strong AQP1 immunofluorescence was detected within the vascular endothelium in both freshwater (FW)- and SW-acclimated eels and in the epithelial apical brush border in the posterior/ rectal gut regions of SW-acclimated eels. Cortisol infusion into FW eels had no effect on intestinal AQPe mRNA expression but induced increases in AQP1 mRNA and protein levels. These results provide evidence for the presence of a SW-induced and steroid-regulated AQP water channel pathway within the intestine of the European eel.

AB - Complementary DNAs encoding homologs of the mammalian aqua-glyceroporins (termed AQPe) and aquaporin-1 isoforms (termed AQP1) were isolated from the European eel. The AQP amino acid sequences share 35 - 54% identity with other known human AQPs. Although AQPe mRNA expression was approximately equivalent along the entire length of the gut, AQP1 expression was the highest in the posterior/rectal segment. Seawater ( SW) acclimation increased AQP1 mRNA abundance by 5- and 17-fold in the anterior, 14- and 23-fold in the mid-, and 9- and 17-fold in the posterior/ rectal gut regions of yellow and silver eels, respectively. SW acclimation had an effect on AQPe mRNA expression only in the midintestine of silver eels, where a small but significant 1.7-fold increase in abundance was measured. Western blots using an eel AQP1-specific antibody identified the presence of a major immunoreactive 28-kDa protein, primarily within the posterior/ rectal segment. A 3-wk SW transfer induced an increase in AQP1 protein abundance in all intestinal segments, with the posterior/ rectal region still expressing protein levels ∼ 40- and 8-fold higher than the anterior and midsegments, respectively. Strong AQP1 immunofluorescence was detected within the vascular endothelium in both freshwater (FW)- and SW-acclimated eels and in the epithelial apical brush border in the posterior/ rectal gut regions of SW-acclimated eels. Cortisol infusion into FW eels had no effect on intestinal AQPe mRNA expression but induced increases in AQP1 mRNA and protein levels. These results provide evidence for the presence of a SW-induced and steroid-regulated AQP water channel pathway within the intestine of the European eel.

KW - Anguilla anguilla

KW - gastrointestinal tract

KW - AQP1

KW - AQPe

KW - corticosteroid

KW - ANGUILLA-ANGUILLA

KW - WATER TRANSPORT

KW - GASTROINTESTINAL-TRACT

KW - GLANDULAR TISSUES

KW - GENE-EXPRESSION

KW - COMPLEX TISSUES

KW - MESSENGER-RNA

KW - JAPONICA

KW - ABSORPTION

KW - CHANNELS

UR - http://www.scopus.com/inward/record.url?scp=19344373580&partnerID=8YFLogxK

U2 - 10.1152/ajpregu.00747.2004

DO - 10.1152/ajpregu.00747.2004

M3 - Article

VL - 288

SP - R1733-R1743

JO - American Journal of Physiology. Regulatory, Integrative and Comparative Physiology

T2 - American Journal of Physiology. Regulatory, Integrative and Comparative Physiology

JF - American Journal of Physiology. Regulatory, Integrative and Comparative Physiology

SN - 0363-6119

IS - 6

ER -

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ID: 339262