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Ring nucleases deactivate Type III CRISPR ribonucleases by degrading cyclic oligoadenylate

Research output: Contribution to journalArticle

Author(s)

Januka S Athukoralage, Christophe Rouillon, Shirley Graham, Sabine Grüschow, Malcolm F White

School/Research organisations

Abstract

The CRISPR system provides adaptive immunity against mobile genetic
elements in prokaryotes, using small CRISPR RNAs that direct effector
complexes to degrade invading nucleic acids1,2,3. Type III effector complexes were recently demonstrated to synthesize a  novel second messenger, cyclic oligoadenylate, on binding target RNA4,5. Cyclic oligoadenylate, in turn, binds to and activates ribonucleases  and other factors—via a CRISPR-associated Rossman-fold domain—and thereby induces in the cell an antiviral state that is important for immunity. The mechanism of the ‘off-switch’ that resets the system is not understood. Here we identify the nuclease that degrades these cyclic oligoadenylate ring molecules. This ‘ring nuclease’ is itself a protein of the CRISPR-associated Rossman-fold family, and has a metal-independent mechanism that cleaves cyclic tetraadenylate rings to generate linear diadenylate species and switches off the antiviral state. The identification of ring nucleases adds an important insight tothe CRISPR system.
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Details

Original languageEnglish
Pages (from-to)277-280
Number of pages16
JournalNature
Volume562
Issue number7726
Early online date19 Sep 2018
DOIs
Publication statusPublished - 11 Oct 2018

    Research areas

  • Archaeon sulfolobus-solfataricus, CAS systems, Image-analysis, Mechanism, Classification, Discovery, Immunity, RNA

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