Skip to content

Research at St Andrews

RNAseq analysis of fast skeletal muscle in restriction-fed transgenic coho salmon (Oncorhynchus kisutch): an experimental model uncoupling the growth hormone and nutritional signals regulating growth

Research output: Contribution to journalArticle


Coho salmon (Oncorhynchus kisutch) transgenic for growth hormone (Gh) express Gh in multiple tissues which results in increased appetite and continuous high growth with satiation feeding. Restricting Gh-transgenics to the same lower ration (TR) as wild-type fish (WT) results in similar growth, but with the recruitment of fewer, larger diameter, muscle skeletal fibres to reach a given body size. In order to better understand the genetic mechanisms behind these different patterns of muscle growth and to investigate how the decoupling of Gh and nutritional signals affects gene regulation we used RNA-seq to compare the fast skeletal muscle transcriptome in TR and WT coho salmon.
Illumina sequencing of individually barcoded libraries from 6 WT and 6 TR coho salmon yielded 704,550,985 paired end reads which were used to construct 323,115 contigs containing 19,093 unique genes of which >10,000 contained >90 % of the coding sequence. Transcripts coding for 31 genes required for myoblast fusion were identified with 22 significantly downregulated in TR relative to WT fish, including 10 (vaspa, cdh15, graf1, crk, crkl, dock1, trio, plekho1a, cdc42a and dock5) associated with signaling through the cell surface protein cadherin. Nineteen out of 44 (43 %) translation initiation factors and 14 of 47 (30 %) protein chaperones were upregulated in TR relative to WT fish.
TR coho salmon showed increased growth hormone transcripts and gene expression associated with protein synthesis and folding than WT fish even though net rates of protein accretion were similar. The uncoupling of Gh and amino acid signals likely results in additional costs of transcription associated with protein turnover in TR fish. The predicted reduction in the ionic costs of homeostasis in TR fish associated with increased fibre size were shown to involve multiple pathways regulating myotube fusion, particularly cadherin signaling.


Original languageEnglish
Article number564
Number of pages8
JournalBMC Genomics
Issue number15
Publication statusPublished - 31 Jul 2015

    Research areas

  • Salmon, Growth, Teleost fish, Growth hormone transgenics, Skeletal muscle transcriptome, Fish nutrition

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Influence of feed ration size on somatic and muscle growth in landlocked dwarf and farmed Atlantic salmon Salmo salar

    Andersen, Ø., Vieira, V., Dessen, J-E. & Johnston, I. A., Apr 2019, In : Journal of Fish Biology. 94, 4, p. 614-620 7 p.

    Research output: Contribution to journalArticle

  2. Genomic tools and selective breeding in molluscs

    Hollenbeck, C. M. & Johnston, I. A., 18 Jul 2018, In : Frontiers in Genetics. 9, 15 p., 253.

    Research output: Contribution to journalReview article

  3. A collaborative European approach to accelerating translational marine science

    Brennecke, P., Ferrante, M., Johnston, I. A. & Smith, D., 5 Jul 2018, In : Journal of Marine Science and Engineering. 6, 3, 12 p., 81.

    Research output: Contribution to journalArticle

  4. A workflow used to design low density SNP panels for parentage assignment and traceability in aquaculture species and its validation in Atlantic salmon

    Holman, L. E., Garcia de la Serrana, D., Onoufriou, A., Hillestad, B. & Johnston, I. A., 1 Jul 2017, In : Aquaculture. 476, p. 59-64 6 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Genetic mapping and comparative genomics to inform restoration enhancement and culture of southern flounder, Paralichthys lethostigma

    O’Leary, S. J., Hollenbeck, C. M., Vega, R. R., Gold, J. R. & Portnoy, D. S., 23 Feb 2018, In : BMC Genomics. 19, 11 p., 163.

    Research output: Contribution to journalArticle

  2. STATAWAARS: a promoter motif associated with spatial expression in the major effector-producing tissues of the plant-parasitic nematode Bursaphelenchus xylophilus

    Espada, M., Eves-van den Akker, S., Maier, T., Vijayapalani, P., Baum, T., Mota, M. & Jones, J. T., 27 Jul 2018, In : BMC Genomics. 19, 553.

    Research output: Contribution to journalArticle

  3. Comparative transcriptomics and proteomics of three different aphid species identifies core and diverse effector sets

    Thorpe, P., Cock, P. J. A. & Bos, J., 2 Mar 2016, In : BMC Genomics. 17, 18 p., 172.

    Research output: Contribution to journalArticle

  4. The Ess/Type VII secretion system of Staphylococcus aureus shows unexpected genetic diversity

    Warne, B., Harkins, C. P., Harris, S. R., Vatsiou, A., Stanley-Wall, N., Parkhill, J., Peacock, S. J., Palmer, T. & Holden, M. T. G., 13 Mar 2016, In : BMC Genomics. 17, 1, p. 222

    Research output: Contribution to journalArticle

ID: 207292055