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Sharpening emitter localization in front of a tuned mirror

Research output: Contribution to journalArticle

Author(s)

Hannah S. Heil, Benjamin Schreiber, Ralph Götz, Monika Emmerling, Marie-Christine Dabauvalle, Georg Krohne, Sven Höfling, Martin Kamp, Markus Sauer, Katrin G. Heinze

School/Research organisations

Abstract

Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tunedmirror2–4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor oftwo. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.
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Details

Original languageEnglish
Article number99
Number of pages8
JournalLight: Science & Applications
Volume7
DOIs
Publication statusPublished - 5 Dec 2018

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