Skip to content

Research at St Andrews

Structure and mechanism of the CMR complex for CRISPR-Mediated antiviral immunity

Research output: Contribution to journalArticlepeer-review

Author(s)

Jing Zhang, Christophe Rouillon, Melina Kerou, Judith Reeks, Kim Brugger, Shirley Graham, Julia Reimann, Giuseppe Cannone, Huanting Liu, Sonja-Verena Albers, James H. Naismith, Laura Spagnolo, Malcolm F White

School/Research organisations

Abstract

The prokaryotic clusters of regularly interspaced palindromic repeats (CRISPR) system utilizes genomically encoded CRISPR RNA (crRNA), derived from invading viruses and incorporated into ribonucleoprotein complexes with CRISPR-associated (CAS) proteins, to target and degrade viral DNA or RNA on subsequent infection. RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse "payload" of targeting crRNA. The crystal structure of Cmr7 and low-resolution structure of the complex are presented. S. solfataricus CMR cleaves RNA targets in an endo-nucleolytic reaction at UA dinucleotides. This activity is dependent on the 8 nt repeat-derived 5' sequence in the crRNA, but not on the presence of a proto-spacer-associated motif (PAM) in the target. Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets.

Close

Details

Original languageEnglish
Pages (from-to)303-313
Number of pages11
JournalMolecular Cell
Volume45
Issue number3
DOIs
Publication statusPublished - 10 Feb 2012

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. The CRISPR ancillary effector Can2 is a dual-specificity nuclease potentiating type III CRISPR defence

    Zhu, W., McQuarrie, S. J., Gruschow, S., McMahon, S., Graham, S., Gloster, T. & White, M., 15 Feb 2021, In: Nucleic Acids Research. Advance Article, 13 p., gkab073.

    Research output: Contribution to journalArticlepeer-review

  2. Facile and scalable expression and purification of transcription factor IIH (TFIIH) core complex

    Sanles-Falagan, R., Petrovic-Stojanovska, B. & White, M. F., Oct 2020, In: Protein Expression and Purification. 174, 105660.

    Research output: Contribution to journalArticlepeer-review

  3. Tetramerisation of the CRISPR ring nuclease Crn3/Csx3 facilitates cyclic oligoadenylate cleavage

    Athukoralage, J. S., McQuarrie, S. J., Gruschow, S., Graham, S., Gloster, T. & White, M., 20 Jul 2020, In: eLife. 9, 19 p., e57627.

    Research output: Contribution to journalArticlepeer-review

  4. Bacteria SAVED from viruses

    White, M. F., 9 Jul 2020, In: Cell. 182, 1, p. 5-6 2 p.

    Research output: Contribution to journalArticlepeer-review

  5. Fuse to defuse: a self-limiting ribonuclease-ring nuclease fusion for type III CRISPR defence

    Samolygo, A., Athukoralage, J. S., Graham, S. & White, M., 19 Jun 2020, In: Nucleic Acids Research. 48, 11, p. 6149–6156 8 p.

    Research output: Contribution to journalArticlepeer-review

Related by journal

  1. DNA damage-induced nucleosome depletion enhances homology search independently of local break movement

    Cheblal, A., Challa, K., Seeber, A., Shimada, K., Yoshida, H., Ferreira, H. C., Amitai, A. & Gasser, S. M., 15 Oct 2020, In: Molecular Cell. 80

    Research output: Contribution to journalArticlepeer-review

  2. Non-canonical activation of the DNA sensing adaptor STING by ATM and IFI16 mediates NF-κB signalling after nuclear DNA damage

    Dunphy, G., Flannery, S. M., Almine, J. F., Connolly, D. J., Paulus, C., Jonsson, K. L., Jakobsen, M. R., Nevels, M. M., Bowie, A. G. & Unterholzner, L., 6 Sep 2018, In: Molecular Cell. 71, 5, p. 745-760 22 p., e5.

    Research output: Contribution to journalArticlepeer-review

  3. Structure of the CRISPR Interference complex CSM reveals key similarities with Cascade

    Rouillon, C., Zhou, M., Zhang, J., Argyris, P., Beilsten-Edmands, V., Cannone, G., Graham, S., Robinson, C., Spagnolo, L. & White, M. F., 10 Oct 2013, In: Molecular Cell. 52, 1, p. 124-134 11 p.

    Research output: Contribution to journalArticlepeer-review

  4. The Histone Chaperones Nap1 and Vps75 Bind Histones H3 and H4 in a Tetrameric Conformation

    Bowman, A., Ward, R., Wiechens, N., Singh, V., El Mkami, H., Norman, D. G. & Owen-Hughes, T., 18 Feb 2011, In: Molecular Cell. 41, 4, p. 398-408 11 p.

    Research output: Contribution to journalArticlepeer-review

ID: 18094393

Top