Skip to content

Research at St Andrews

Structure of the DNA repair helicase XPD

Research output: Contribution to journalArticle


Huanting Liu, Jana Rudolf, K A Johnson, Stephen McMahon, Muse Oke, L Carter, A-M McRobbie, S E Brown, James Henderson Naismith, Malcolm Frederick White

School/Research organisations


The XPD helicase (Rad3 in Saccharomyces cerevisiae) is a component of transcription factor IIH (TFIIH), which functions in transcription initiation and Nucleotide Excision Repair in eukaryotes, catalyzing DNA duplex opening localized to the transcription start site or site of DNA damage, respectively. XPD has a 50 to 30 polarity and the helicase activity is dependent on an iron-sulfur cluster binding domain, a feature that is conserved in related helicases such as FancJ. The xpd gene is the target of mutation in patients with xeroderma pigmentosum, trichothiodystrophy, and Cockayne's syndrome, characterized by a wide spectrum of symptoms ranging from cancer susceptibility to neurological and developmental defects. The 2.25 angstrom crystal structure of XPD from the crenarchaeon Sulfolobus tokodaii, presented here together with detailed biochemical analyses, allows a molecular understanding of the structural basis for helicase activity and explains the phenotypes of xpd mutations in humans.



Original languageEnglish
Pages (from-to)801-812
Number of pages12
Issue number5
StatePublished - 30 May 2008

    Research areas


Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Ring nucleases deactivate Type III CRISPR ribonucleases by degrading cyclic oligoadenylate

    Athukoralage, J. S., Rouillon, C., Graham, S., Grüschow, S. & White, M. F. 11 Oct 2018 In : Nature. 562, 7726, p. 277-280 16 p.

    Research output: Contribution to journalArticle

  2. Control of cyclic oligoadenylate synthesis in a type III CRISPR system

    Rouillon, C., Athukoralage, J. S., Graham, S., Grüschow, S. & White, M. F. 19 Jul 2018 In : eLife. 7, 22 p., e36734

    Research output: Contribution to journalArticle

  3. DNA repair in the Archaea – an emerging picture

    White, M. F. & Allers, T. Jul 2018 In : FEMS Microbiology Reviews. 42, 4, p. 514-526 13 p.

    Research output: Contribution to journalReview article

  4. Prespacer processing and specific integration in a Type I-A CRISPR system

    Rollie, C., Graham, S., Rouillon, C. & White, M. F. 16 Feb 2018 In : Nucleic Acids Research. 46, 3, p. 1007-1020 14 p.

    Research output: Contribution to journalArticle

  5. DNA‐Interacting characteristics of the archaeal Rudiviral protein SIRV2_Gp1

    Peeters, E., Boon, M., Rollie, C. J. C., Willaert, R. G., Voet, M., White, M. F., Prangishvili, D., Lavigne, R. & Quax, T. E. F. Jul 2017 In : Viruses. 9, 7, 13 p., 190

    Research output: Contribution to journalArticle

Related by journal

  1. FUS phase separation is modulated by a molecular chaperone and methylation of arginine cation-π interactions

    Qamar, S. , Wang, G. , Randle, S. J. , Ruggeri, F. S. , Varela, J. A. , Lin, J. Q. , Phillips, E. C. , Miyashita, A. , Williams, D. , Ströhl, F. , Meadows, W. , Ferry, R. , Dardov, V. J. , Tartaglia, G. G. , Farrer, L. A. , Kaminski Schierle, G. S. , Kaminski, C. F. , Holt, C. E. , Fraser, P. E. , Schmitt-Ulms, G. & 4 others Klenerman, D., Knowles, T., Vendruscolo, M. & St George-Hyslop, P. 19 Apr 2018 In : Cell. 173, 3, p. 720-734 e15

    Research output: Contribution to journalArticle

  2. Oligopeptide signaling through TbGPR89 drives trypanosome quorum sensing

    Rojas, F., Silvester, E., Young, J., Milne, R., Tettey, M., Houston, D. R., Walkinshaw, M. D., Pérez-Pi, I., Auer, M., Denton, H., Smith, T. K., Thompson, J. & Matthews, K. R. 29 Nov 2018 In : Cell. 176, 1-2, p. 306-317 12 p., e16

    Research output: Contribution to journalArticle

  3. Timing the landmark events in the evolution of clear cell renal cell cancer: TRACERx renal

    Mitchell, T. J. , Turajlic, S. , Rowan, A. , Nicol, D. , Farmery, J. H. R. , O’Brien, T. , Martincorena, I. , Tarpey, P. , Angelopoulos, N. , Yates, L. R. , Butler, A. P. , Raine, K. , Stewart, G. D. , Challacombe, B. , Fernando, A. , Lopez, J. I. , Hazell, S. , Chandra, A. , Chowdhury, S. , Rudman, S. & 27 others Soultati, A., Stamp, G., Fotiadis, N., Pickering, L., Au, L., Spain, L., Lynch, J., Stares, M., Teague, J., Maura, F., Wedge, D. C., Horswell, S., Chambers, T., Litchfield, K., Xu, H., Stewart, A., Elaidi, R., Oudard, S., McGranahan, N., Csabai, I., Gore, M., Futreal, P. A., Larkin, J., Lynch, A. G., Szallasi, Z., Swanton, C. & Campbell, P. J. 19 Apr 2018 In : Cell. 173, 3, p. 611-623 13 p., e17

    Research output: Contribution to journalArticle

  4. Cryo-EM structure of a relaxase reveals the molecular basis of DNA unwinding during bacterial conjugation

    Ilangovan, A., Kay, C. W. M., Roier, S., El Mkami, H., Salvadori, E., Zechner, E. L., Zanetti, G. & Waksman, G. 4 May 2017 In : Cell. 169, 4, p. 708-721 27 p., e12

    Research output: Contribution to journalArticle

  5. Structure of the bacterial sex F pilus reveals an assembly of a stoichiometric protein-phospholipid complex

    Costa, T. R. D., langovan, A. I., Ukleja, M., Redjez, A., Santini, J. M., Smith, T. K., Egelman, E. H. & Waksman, G. 8 Sep 2016 In : Cell. 166, 6, p. 1436-1444 20 p., e10

    Research output: Contribution to journalArticle

ID: 424508