Skip to content

Research at St Andrews

Subcellular localisation of Theiler's Murine Encephalomyelitis Virus (TMEV) capsid subunit VP1 vis-á-vis host protein Hsp90

Research output: Contribution to journalArticlepeer-review

Author(s)

Caroline Ross, Nicole Upfold, Garry A. Luke, Özlem Tastan Bishop, Caroline Knox

School/Research organisations

Abstract

The VP1 subunit of the picornavirus capsid is the major antigenic determinant and mediates host cell attachment and virus entry. To investigate the localisation of Theiler's murine encephalomyelitis virus (TMEV) VP1 during infection, a bioinformatics approach was used to predict a surface-exposed, linear epitope region of the protein for subsequent expression and purification. This region, comprising the N-terminal 112 amino acids of the protein, was then used for rabbit immunisation, and the resultant polyclonal antibodies were able to recognise full length VP1 in infected cell lysates by Western blot. Following optimisation, the antibodies were used to investigate the localisation of VP1 in relation to Hsp90 in infected cells by indirect immunofluorescence and confocal microscopy. At 5 hours post infection, VP1 was distributed diffusely in the cytoplasm with strong perinuclear staining but was absent from the nucleus of all cells analysed. Dual-label immunofluorescence using anti-TMEV VP1 and anti-Hsp90 antibodies indicated that the distribution of both proteins colocalised in the cytoplasm and perinuclear region of infected cells. This is the first report describing the localisation of TMEV VP1 in infected cells, and the antibodies produced provide a valuable tool for investigating the poorly understood mechanisms underlying the early steps of picornavirus assembly.
Close

Details

Original languageEnglish
Pages (from-to)53-63
JournalVirus Research
Volume222
Early online date3 Jun 2016
DOIs
Publication statusPublished - 15 Aug 2016

    Research areas

  • Theiler's murine encephalomyelitis virus, VP1, Hsp90, Polyclonal antibody, Capsid

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Expression and immunogenicity of secreted forms of bovine ephemeral fever virus glycoproteins applied to subunit vaccine development

    Lo, Y-T., Tulloch, F., Wu, H-C., Luke, G. A., Ryan, M. D. & Chu, C-Y., 3 Mar 2021, In: Journal of Applied Microbiology. Early View

    Research output: Contribution to journalArticlepeer-review

  2. Occurrence of human enteric viruses in water sources and shellfish: a focus on Africa

    Upfold, N. S., Luke, G. A. & Knox, C., 27 Jan 2021, In: Food and Environmental Virology. 13, 1, p. 1-31 31 p.

    Research output: Contribution to journalReview articlepeer-review

  3. Translation of Viral Proteins

    Ryan, M. D. & Luke, G. A., 2021, Encyclopedia of Virology: The Virus as a Concept - Fundamentals of Virology. Bamford, D. & Zuckerman, M. (eds.). 4 ed. New York: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, Vol. 1. p. 444-459 15 p.

    Research output: Chapter in Book/Report/Conference proceedingChapter

  4. A transgenic line that reports CSF1R protein expression provides a definitive marker for the mouse mononuclear phagocyte system

    Grabert, K., Sehgal, A., Irvine, K. M., Wollscheid-Lengeling, E., Ozdemir, D. D., Stables, J., Luke, G. A., Ryan, M. D., Adamson, A., Humphreys, N. E., Sandrock, C. J., Rojo, R., Verkasalo, V. A., Mueller, W., Hohenstein, P., Pettit, A. R., Pridans, C. & Hume, D. A., 1 Dec 2020, In: The Journal of Immunology. 205, 11

    Research output: Contribution to journalArticlepeer-review

  5. The first detection of Human Bocavirus Species 2 and 3 in raw sewage and mussels in South Africa

    Onosi, O., Upfold, N., Jukes, M. D., Luke, G. A. & Knox, C. M., 1 Mar 2020, In: Food and Environmental Virology. 12, p. 84-88

    Research output: Contribution to journalArticlepeer-review

Related by journal

  1. Virus Research (Journal)

    Richard Michael Elliott (Member of editorial board)

    20022006

    Activity: Publication peer-review and editorial work typesEditor of research journal

Related by journal

  1. RNA sequence analysis of diseased groundnut (Arachis hypogaea) reveals the full genome of groundnut rosette assistor virus (GRAV)

    Jones, S., Cowan, G., MacFarlane, S., Mukoye, B., Mangeni, B. C., Were, H. & Torrance, L., Feb 2020, In: Virus Research. 277, 197837.

    Research output: Contribution to journalArticlepeer-review

  2. Influenza virus activation of the interferon system

    Killip, M. J., Fodor, E. & Randall, R. E., 2015, In: Virus Research.

    Research output: Contribution to journalArticlepeer-review

  3. Heat shock protein 40 (Hsp40) plays a key role in the virus life cycle

    Knox, C., Luke, G. A., Blatch, G. L. & Pesce, E-R., Sep 2011, In: Virus Research. 160, 1-2, p. 15-24 10 p.

    Research output: Contribution to journalReview articlepeer-review

ID: 243242418

Top