Skip to content

Research at St Andrews

Tandem affinity purification of exosome and replication factor C complexes from the non-human infectious kinetoplastid parasite Crithidia fasciculata

Research output: Contribution to journalArticle

Author(s)

School/Research organisations

Abstract

Kinetoplastid parasites are responsible for a range of diseases with significant global impact. Trypanosoma brucei and Trypanosoma cruzi cause human African trypanosomiasis and Chagas disease, respectively, while various Leishmania species are responsible for cutaneous, mucocutaneous and visceral leishmaniasis. Understanding the biology of these organisms is key for effective diagnosis, prophylaxis and treatment. The insect parasite Crithidia fasciculata offers a safe and low-cost alternative for studies of kinetoplastid biology. C. fasciculata does not infect humans, can be cultured to high yields in inexpensive serum-free medium in a standard laboratory, and has a completely sequenced publically available genome. Taking advantage of these features, however, requires the adaptation of existing methods of analysis to C. fasciculata. Tandem affinity purification is a widely used method that allows for the rapid purification of intact protein complexes under native conditions. Here we report the application of tandem affinity purification to C. fasciculata for the first time, demonstrating the effectiveness of the technique by purifying both the intact exosome and replication factor C complexes. Adding tandem affinity purification to the C. fasciculata toolbox significantly enhances the utility of this excellent model system.
Close

Details

Original languageEnglish
Pages (from-to)19-22
Number of pages4
JournalMolecular and Biochemical Parasitology
Volume217
Early online date24 Aug 2017
DOIs
StatePublished - Oct 2017

    Research areas

  • Crithidia fasciculata, Tandem affinity purification, Kinetoplastid, Exosome, DNA replication

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Branched late-steps of the cytosolic iron-sulphur cluster assembly machinery of Trypanosoma brucei

    Tonini, M. L., Peña-Diaz, P., Haindrich, A. C., Basu, S., Kriegová, E., Pierik, A. J., Lill, R., MacNeill, S. A., Smith, T. K. & Lukeš, J. 22 Oct 2018 In : PLoS Pathogens. 14, 10, 31 p., e1007326

    Research output: Contribution to journalArticle

  2. Biological evaluation and structure activity relationship of 9-methyl-1-phenyl-9H-pyrido[3,4-b]indole derivatives as anti-leishmanial agents

    Ashok, P., Chander, S., Smith, T. K., Prakash Singh, R., Nath Jha, P. & Sankaranarayanan, M. 22 Nov 2018 In : Bioorganic Chemistry. 84, p. 98-105

    Research output: Contribution to journalArticle

  3. Oligopeptide signaling through TbGPR89 drives trypanosome quorum sensing

    Rojas, F., Silvester, E., Young, J., Milne, R., Tettey, M., Houston, D. R., Walkinshaw, M. D., Pérez-Pi, I., Auer, M., Denton, H., Smith, T. K., Thompson, J. & Matthews, K. R. 29 Nov 2018 In : Cell. 176, 1-2, p. 306-317 12 p., e16

    Research output: Contribution to journalArticle

Related by journal

  1. Molecular and Biochemical Parasitology (Journal)

    Smith, T. K. (Member of editorial board)
    2015 → …

    Activity: Publication peer-review and editorial workPeer review of manuscripts

Related by journal

  1. NADH dehydrogenase of Trypanosoma brucei is important for efficient acetate production in bloodstream forms

    Surve, S. V., Jensen, B. C., Heestand, M., Mazet, M., Smith, T. K., Bringaud, F., Parsons, M. & Schnaufer, A. Jan 2017 In : Molecular and Biochemical Parasitology. 211, p. 57-61 5 p.

    Research output: Contribution to journalArticle

  2. Lipid metabolism in Trypanosoma brucei

    Smith, T. K. & Buetikofer, P. Aug 2010 In : Molecular and Biochemical Parasitology. 172, 2, p. 66-79 14 p.

    Research output: Contribution to journalReview article

  3. Expressed sequence tag (EST) analysis of the pine wood nematode Bursaphelenchus xylophilus and B-mucronatus

    Kikuchi, T., Aikawa, T., Kosaka, H., Pritchard, L., Ogura, N. & Jones, J. T. Sep 2007 In : Molecular and Biochemical Parasitology. 155, 1, p. 9-17 9 p.

    Research output: Contribution to journalArticle

ID: 250869320