Skip to content

Research at St Andrews

The DNA repair helicases XPD and FancJ have essential iron-sulfur domains

Research output: Contribution to journalArticle

Abstract

DNA helicases are essential components of the cellular machinery for DNA replication, recombination, repair, and transcription. The XPD and FancJ proteins are related helicases involved in the nucleotide excision repair (NER) and Fanconi anemia repair pathways, respectively. We demonstrate that both proteins have a conserved domain near the N terminus that includes an iron-sulfur (Fe-S) cluster. Three absolutely conserved cysteine residues provide ligands for the Fe-S cluster, which is essential for the helicase activity of XPD. Yeast strains harboring mutations in the Fe-S domain of Rad3 (yeast XPD) are defective in excision repair of UV photoproducts. Clinically relevant mutations in patients with trichothiodystrophy (TTD) and Fanconi anemia disrupt the Fe-S clusters of XPD and FancJ and thereby abolish helicase activity.

Close

Details

Original languageEnglish
Pages (from-to)801-808
Number of pages8
JournalMolecular Cell
Volume23
Issue number6
DOIs
StatePublished - 15 Sep 2006

    Research areas

  • ENZYME ENDONUCLEASE-III, SACCHAROMYCES-CEREVISIAE, ESCHERICHIA-COLI, XERODERMA-PIGMENTOSUM, FACTOR TFIIH, PROTEIN, GENE, TRANSCRIPTION, TRICHOTHIODYSTROPHY, BACH1

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba

    Jelinska, C., Petrovic-Stojanovska, B., Ingledew, W. J. & White, M. F. 1 Apr 2010 In : Biochemical Journal. 427, 1, p. 49-55 7 p.

    Research output: Contribution to journalArticle

  2. Control of cyclic oligoadenylate synthesis in a type III CRISPR system

    Rouillon, C. M. J., Athukoralage, J. S., Graham, S., Gruschow, S. & White, M. F. 19 Jul 2018 In : eLife. 7, 22 p., e36734

    Research output: Contribution to journalArticle

  3. DNA repair in the Archaea – an emerging picture

    White, M. F. & Allers, T. 5 May 2018 In : FEMS Microbiology Reviews. Advance articles

    Research output: Contribution to journalReview article

  4. Prespacer processing and specific integration in a Type I-A CRISPR system

    Rollie, C., Graham, S., Rouillon, C. & White, M. F. 16 Feb 2018 In : Nucleic Acids Research. 46, 3, p. 1007-1020 14 p.

    Research output: Contribution to journalArticle

  5. DNA‐Interacting characteristics of the archaeal Rudiviral protein SIRV2_Gp1

    Peeters, E., Boon, M., Rollie, C. J. C., Willaert, R. G., Voet, M., White, M. F., Prangishvili, D., Lavigne, R. & Quax, T. E. F. Jul 2017 In : Viruses. 9, 7, 13 p., 190

    Research output: Contribution to journalArticle

Related by journal

  1. Structure of the CRISPR Interference complex CSM reveals key similarities with Cascade

    Rouillon, C., Zhou, M., Zhang, J., Argyris, P., Beilsten-Edmands, V., Cannone, G., Graham, S., Robinson, C., Spagnolo, L. & White, M. F. 10 Oct 2013 In : Molecular Cell. 52, 1, p. 124-134 11 p.

    Research output: Contribution to journalArticle

  2. Structure and mechanism of the CMR complex for CRISPR-Mediated antiviral immunity

    Zhang, J., Rouillon, C., Kerou, M., Reeks, J., Brugger, K., Graham, S., Reimann, J., Cannone, G., Liu, H., Albers, S-V., Naismith, J. H., Spagnolo, L. & White, M. F. 10 Feb 2012 In : Molecular Cell. 45, 3, p. 303-313 11 p.

    Research output: Contribution to journalArticle

  3. The Histone Chaperones Nap1 and Vps75 Bind Histones H3 and H4 in a Tetrameric Conformation

    Bowman, A., Ward, R., Wiechens, N., Singh, V., El Mkami, H., Norman, D. G. & Owen-Hughes, T. 18 Feb 2011 In : Molecular Cell. 41, 4, p. 398-408 11 p.

    Research output: Contribution to journalArticle

  4. ChlR1 is required for loading papillomavirus E2 onto mitotic chromosomes and viral genome maintenance.

    Parish, J. L., Bean, A. M., Park, R. B. & Androphy, E. J. 28 Dec 2006 In : Molecular Cell. 24, 6, p. 867-876 10 p.

    Research output: Contribution to journalArticle

  5. Direct observation of DNA distortion by the RSC complex

    Lia, G., Praly, E., Ferreira, H., Stockdale, C., Tse-Dinh, Y. C., Dunlap, D., Croquette, V., Bensimon, D. & Owen-Hughes, T. 3 Feb 2006 In : Molecular Cell. 21, 3, p. 417-425 9 p.

    Research output: Contribution to journalArticle

ID: 323355