Skip to content

Research at St Andrews

The identification and management of pain, suffering and distress in cephalopods, including anaesthesia, analgesia and humane killing

Research output: Contribution to journalArticle

Author(s)

Paul L.R. Andrews, Anne-Sophie Darmaillacq, Ngaire Dennison, Ian G. Gleadall, Penny Hawkins, John B. Messenger, Daniel Osorio, Valerie Jane Smith, Jane A. Smith

School/Research organisations

Abstract

From January 2013 scientific projects involving cephalopods became regulated by Directive 2010/63/EU, but at present there is little guidance specifically for cephalopods on a number of key requirements of the Directive, including: recognition of pain, suffering and distress and implementation of humane end-points; anaesthesia and analgesia, and humane killing. This paper critically reviews these key areas prior to the development of guidelines and makes recommendations, including identifying topics for further research. In particular: a) Evidence on how cephalopods might experience pain is reviewed; and a draft scheme of behavioural and physiological criteria for recognising and assessing pain, suffering and distress in cephalopods used in scientific procedures is presented and discussed. b) Agents and protocols currently used for general anaesthesia and analgesia are evaluated. Magnesium chloride, ethanol and clove oil are themost frequently used agents, but their efficacy and potential for induction of aversion need to be systematically investigated, according to the species of cephalopod and factors such as body weight, sex and water temperature. Means of sedating animals prior to anaesthesia should be investigated. Criteria for assessing depth of anaesthesia, including depression of ventilation, decrease in chromatophore tone (paling), reduced arm activity, tone and sucker adhesiveness, loss of normal posture and righting reflex, and loss
of response to a noxious stimulus, are discussed. c) Analgesia should be provided for cephalopods used in scientific procedures, whenever this would be the case for vertebrates. However, research is needed to evaluate effective
agents and administration routes for cephalopods. d) Techniques for local anaesthesia need to be defined and evaluated. e) Currently used methods of killing and criteria for confirmation of death in cephalopods are evaluated.
Based on present knowledge, a protocol for humane killing of cephalopods is proposed. However, further evaluation is needed, along with development of humane methods of killing that will not compromise study of the brain.
On humane grounds: (i). mechanical (as opposed to chemical) methods of killing should not be used on conscious cephalopods (unless specifically authorised by the national competent authority); and (ii). hatchlings and larvae should be killed by overdose of anaesthetic and not by immersion in tissue fixative. Key gaps in current knowledge are also highlighted, so as to encourage research that will contribute to the evidence base needed to develop guidelines to the Directive.
Close

Details

Original languageEnglish
Pages (from-to)46-64
Number of pages18
JournalJournal of Experimental Marine Biology and Ecology
Volume447
Early online date14 Aug 2013
DOIs
Publication statusPublished - Sep 2013

    Research areas

  • Anaesthesia, Analgesia, Cuttlefish, Nociception, Octopus, Pain

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Cellular stress responses to chronic heat shock and shell damage in temperate Mya truncata

    Sleight, V. A., Peck, L. S., Dyrynda, E. A., Smith, V. J. & Clark, M. S., 12 May 2018, In : Cell Stress and Chaperones. First Online, 15 p.

    Research output: Contribution to journalArticle

  2. Wind turbines cause chronic stress in badgers (Meles meles) in Great Britain

    Agnew, R., Smith, V. J. & Fowkes, R., Jul 2016, In : Journal of Wildlife Diseases. 52, 3, p. 459-467 9 p.

    Research output: Contribution to journalArticle

  3. Hematopoiesis and hemocytes in pancrustacean and molluscan models

    Smith, V. J., Accorsi, A. & Malagoli, D., 20 May 2016, The Evolution of the Immune System: Conservation and Diversification. Malagoli, D. (ed.). 1st ed. Academic Press/Elsevier , p. 1-28

    Research output: Chapter in Book/Report/Conference proceedingChapter

  4. Immunology of invertebrates: Cellular

    Smith, V. J., 15 Feb 2016, Encyclopedia of Life Sciences: Wiley. Wiley, 13 p. (Encyclopedia of Life Sciences).

    Research output: Chapter in Book/Report/Conference proceedingChapter

Related by journal

  1. The influence of water motion on the growth rate of the kelp Laminaria digitata

    Kregting, L., Blight, A. J., Elsäßer, B. & Savidge, G., May 2016, In : Journal of Experimental Marine Biology and Ecology. 478, p. 86-95 10 p.

    Research output: Contribution to journalArticle

  2. Indirect effects may buffer negative responses of seagrass invertebrate communities to ocean acidification

    Garrard, S., Gambi, M. C., Scipione, M. B., Lorenti, M., Zupo, V., Paterson, D. M. & Buia, M. C., 2014, In : Journal of Experimental Marine Biology and Ecology. 461, p. 31-38 8 p.

    Research output: Contribution to journalArticle

  3. Modelling harbour seal habitat by combining data from multiple tracking systems

    Bailey, H., Hammond, P. S. & Thompson, P. M., Jan 2014, In : Journal of Experimental Marine Biology and Ecology. 450, p. 30–39

    Research output: Contribution to journalArticle

ID: 204594818