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The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay

Research output: Contribution to journalArticle

Author(s)

Andrea Rachow, Elmar Saathoff, Bariki Mtafya, Daniel Mapamba, Chacha Mangu, Gabriel Rojas-Ponce, Nyanda E. Ntinginya, Martin Boeree, Norbert Heinrich, Stephen H. Gillespie, Michael Hoelscher, PanACEA Consortium

School/Research organisations

Abstract

The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (CTmin). Repeated decontamination was not associated with PCR-inhibition or invalid results. The CTmin of M.tb positive samples was higher in inhibited Xpert runs. Our data indicate that NALC/NaOH-decontamination has an effect on the performance of the Xpert assay, and that CTmin readouts of decontaminated sputum samples should be interpreted with caution.
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Details

Original languageEnglish
JournalTuberculosis
VolumeIn press
Early online date4 Apr 2018
DOIs
Publication statusE-pub ahead of print - 4 Apr 2018

    Research areas

  • Xpert-inhibition, Sputum processing, Quantitative PCR, Xpert-performance

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