Skip to content

Research at St Andrews

The major histocompatibility complex class I immunopeptidome of extracellular vesicles

Research output: Contribution to journalArticlepeer-review

Abstract

Extracellular vesicles (EVs) are released by most cell types and have been associated with multiple immunomodulatory functions. MHC class I molecules have crucial roles in antigen presentation and in eliciting immune responses and are known to be incorporated into EVs. However, the MHC class Iimmunopeptidome of EVs has not been established. Here, using a small-scale immunoisolation of the antigen serotypes HLA-A*02:01 and HLA-B*27:05 expressed on the Epstein–Barr virus–transformed B cell line Jesthom and MS of the eluted peptides from both cells and EVs, we identified 516 peptides that bind either HLA-A*02:01 orHLA-B*27:05. Of importance, the predicted serotype-binding affinities and peptide-anchor motifs did not significantly differ between the peptide pools isolated from cells or EVs,indicating that during EV biogenesis, no obvious editing of the MHC class Iimmunopeptidome occurs. These results, for the first time, establish EVs as a source ofMHC class I peptides that can be used for the study of the immunopeptidome and in the discovery of potential neoantigens for immunotherapies.
Close

Details

Original languageEnglish
Pages (from-to)17084-17092
JournalJournal of Biological Chemistry
Volume292
Early online date31 Aug 2017
DOIs
Publication statusPublished - 13 Oct 2017

    Research areas

  • Extracellular vesicles, Immunology, Major Histocompatibility Complex, Mass spectrometry (MS), Proteomics

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Quantitative proteomic changes in LPS-activated monocyte-derived dendritic cells: a SWATH-MS study

    Arya, S., Wiatrek-Moumoulidis, D., Synowsky, S. A., Shirran, S. L., Botting, C. H., Powis, S. J. & Stewart, A. J., 13 Mar 2019, In: Scientific Reports. 9, 11 p., 4343.

    Research output: Contribution to journalArticlepeer-review

  2. Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration

    Arya, S., Emri, E., Synowsky, S. A., Shirran, S. L., Barzegar-Befroei, N., Peto, T., Botting, C. H., Lengyel, I. & Stewart, A. J., Jul 2018, In: Experimental Eye Research. 172, p. 21-29 9 p.

    Research output: Contribution to journalArticlepeer-review

  3. Adverse effects of Δ9-tetrahydrocannabinol on neuronal bioenergetics during postnatal development

    Beiersdorf, J., Hevesi, Z., Calvigioni, D., Pyszkowski, J., Romanov, R., Szodorai, E., Lubec, G., Shirran, S., Botting, C. H., Kasper, S., Guy, G. W., Gray, R., Di Marzo, V., Harkany, T. & Keimpema, E., 3 Dec 2020, In: JCI Insight. 5, 23, 20 p., e135418.

    Research output: Contribution to journalArticlepeer-review

  4. Life-long epigenetic programming of cortical architecture by maternal ‘Western’ diet during pregnancy

    Cinquina, V., Calvigioni, D., Farlik, M., Halbritter, F., Fife-Gernedl, V., Shirran, S. L., Fuszard, M. A., Botting, C. H., Poullet, P., Piscitelli, F., Máte, Z., Szabo, G., Yanagawa, Y., Kasper, S., Di Marzo, V., Mackie, K., McBain, C., Bock, C., Keimpema, E. & Harkany, T., 18 Nov 2019, In: Molecular Psychiatry. 15 p.

    Research output: Contribution to journalArticlepeer-review

Related by journal

  1. Journal of Biological Chemistry (Journal)

    Simon John Powis (Member of editorial board)

    1 Jun 2015

    Activity: Publication peer-review and editorial work typesEditor of research journal

  2. Journal of Biological Chemistry (Journal)

    Terry K Smith (Member of editorial board)

    2006 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. Iron is a ligand of SecA-like metal-binding domains in vivo

    Cranford-Smith, T., Jamshad, M., Jeeves, M., Chandler, R. A., Yule, J., Robinson, A., Alam, F., Dunne, K. A., Aponte Angarita, E. H., Alanazi, M., Carter, C., Henderson, I. R., Lovett, J. E., Winn, P., Knowles, T. & Huber, D., 2 Apr 2020, In: Journal of Biological Chemistry. Papers in Press

    Research output: Contribution to journalArticlepeer-review

  2. The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex

    Koo, C. Z., Harrison, N., Noy, P. J., Szyroka, J., Matthews, A. L., Hsia, H-E., Müller, S. A., Tüshaus, J., Goulding, J., Willis, K., Apicella, C., Cragoe, B., Davis, E., Keles, M., Malinova, A., McFarlane, T. A., Morrison, P. R., Nguyen, H. T. H., Sykes, M. C., Ahmed, H. & 11 others, Di Maio, A., Seipold, L., Saftig, P., Cull, E., Pliotas, C., Rubinstein, E., Poulter, N. S., Briddon, S. J., Holliday, N. D., Lichtenthaler, S. F. & Tomlinson, M. G., 28 Feb 2020, In: Journal of Biological Chemistry.

    Research output: Contribution to journalArticlepeer-review

  3. Combining SPR with atomic-force microscopy enables singlemolecule insights into activation and suppression of the complement cascade

    Makou, E., Bailey, R. G., Johnston, H., Parkin, J. D., Hulme, A. N., Hähner, G. & Barlow, P. N., 27 Dec 2019, In: Journal of Biological Chemistry. 294, 52, p. 20148-20163 16 p.

    Research output: Contribution to journalArticlepeer-review

  4. Dysregulated Zn2+ homeostasis impairs cardiac type-2 ryanodine receptor and mitsugumin 23 functions, leading to sarcoplasmic reticulum Ca2+ leakage

    Reilly-O'Donnell, B., Robertson, G. B., Karumbi, A., McIntyre, C., Bal, W., Nishi, M., Takeshima, H., Stewart, A. J. & Pitt, S. J., 11 Aug 2017, In: Journal of Biological Chemistry. 292, 32, p. 13361-13373 13 p.

    Research output: Contribution to journalArticlepeer-review

ID: 250989908

Top