Skip to content

Research at St Andrews

The major histocompatibility complex class I immunopeptidome of extracellular vesicles

Research output: Contribution to journalArticle

DOI

Open Access permissions

Open

Abstract

Extracellular vesicles (EVs) are released by most cell types and have been associated with multiple immunomodulatory functions. MHC class I molecules have crucial roles in antigen presentation and in eliciting immune responses and are known to be incorporated into EVs. However, the MHC class Iimmunopeptidome of EVs has not been established. Here, using a small-scale immunoisolation of the antigen serotypes HLA-A*02:01 and HLA-B*27:05 expressed on the Epstein–Barr virus–transformed B cell line Jesthom and MS of the eluted peptides from both cells and EVs, we identified 516 peptides that bind either HLA-A*02:01 orHLA-B*27:05. Of importance, the predicted serotype-binding affinities and peptide-anchor motifs did not significantly differ between the peptide pools isolated from cells or EVs,indicating that during EV biogenesis, no obvious editing of the MHC class Iimmunopeptidome occurs. These results, for the first time, establish EVs as a source ofMHC class I peptides that can be used for the study of the immunopeptidome and in the discovery of potential neoantigens for immunotherapies.
Close

Details

Original languageEnglish
JournalJournal of Biological Chemistry
VolumePapers in Press
Early online date31 Aug 2017
DOIs
StateE-pub ahead of print - 31 Aug 2017

    Research areas

  • Antigen presentation, Extracellular vesicles, MHC class I

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. hSSB1 phosphorylation is dynamically regulated by DNA-PK and PPP-family protein phosphatases

    Ashton, N. W., Paquet, N., Shirran, S. L., Bolderson, E., Kariawasam, R., Touma, C., Fallahbaghery, A., Gamsjaeger, R., Cubeddu, L., Botting, C., Pollock, P. M., O’Byrne, K. J. & Richard, D. J. Jun 2017 In : DNA Repair. 54, p. 30-39 10 p.

    Research output: Contribution to journalArticle

  2. Contaminants in commercial preparations of ‘purified’ small leucine-rich proteoglycans may distort mechanistic studies

    Brown, S. J., Fuller, H. R., Jones, P., Caterson, B., Shirran, S. L., Botting, C. H. & Roberts, S. Feb 2017 In : Bioscience Reports. 37, 1, 14 p.

    Research output: Contribution to journalArticle

  3. Synthesis of next generation maleimide radical labels

    Hajjaj, B., Shah, A., Bell, S., Shirran, S. L., Botting, C. H., Slawin, A. M. Z., Hulme, A. & Lovett, J. E. Oct 2016 In : Synlett. p. 2357-2361 5 p., 27

    Research output: Contribution to journalArticle

  4. Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase

    Shi, X., Botting, C. H., Li, P., Niglas, M., Brennan, B., Shirran, S. L., Szemiel, A. M. & Elliott, R. M. 2 Aug 2016 In : Proceedings of the National Academy of Sciences of the United States of America. 113, 31, p. 8825-8830 6 p.

    Research output: Contribution to journalArticle

  5. Rapid evolution and gene expression: a rapidly-evolving Mendelian trait that silences field crickets has widespread effects on mRNA and protein expression

    Pascoal, S. C. M., Liu, X., Ly, T., Fang, Y., Rockliffe, N., Paterson, S., Shirran, S. L., Botting, C. H. & Bailey, N. W. Jun 2016 In : Journal of Evolutionary Biology. 29, 6, p. 1234-1246 13 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Dysregulated Zn2+ homeostasis impairs cardiac type-2 ryanodine receptor and mitsugumin 23 functions, leading to sarcoplasmic reticulum Ca2+ leakage

    Reilly-O'Donnell, B., Robertson, G. B., Karumbi, A., McIntyre, C., Bal, W., Nishi, M., Takeshima, H., Stewart, A. J. & Pitt, S. J. 11 Aug 2017 In : Journal of Biological Chemistry. 292, 32, p. 13361-13373 13 p.

    Research output: Contribution to journalArticle

  2. Using a genetically encoded sensor to identify inhibitors of Toxoplasma gondii Ca2+ Signalling

    Sidik, S. M., Hortua Triana, M. A., Paul, A. S., El Bakkouri, M., Hackett, C. G., Tran, F., Westwood, N. J., Hui, R., Zuercher, W. J., Duraisingh, M. T., Moreno, S. N. J. & Lourido, S. 29 Apr 2016 In : Journal of Biological Chemistry. 291, 18, p. 9566-9580 15 p.

    Research output: Contribution to journalArticle

  3. Streptococcus pneumoniae NanC. Structural insights into the specificity and mechanism of a sialidase that produces a sialidase inhibitor

    Owen, C. D., Lukacik, P., Potter, J. A., Sleator, O., Taylor, G. L. & Walsh, M. A. 13 Nov 2015 In : Journal of Biological Chemistry. 290, 46, p. 27736-27748 13 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Journal of Biological Chemistry (Journal)

    Powis, S. J. (Member of editorial board)
    1 Jun 2015

    Activity: Publication peer-review and editorial workEditor of research journal

  2. Journal of Biological Chemistry (Journal)

    Smith, T. K. (Member of editorial board)
    2006 → …

    Activity: Publication peer-review and editorial workPeer review of manuscripts

ID: 250989908