Skip to content

Research at St Andrews

The microevolution and epidemiology of Staphylococcus aureus colonization during atopic eczema disease flare

Research output: Contribution to journalArticle

Author(s)

Catriona P. Harkins, Kerry A. Pettigrew, Katarina Oravcová, June Gardner, R. M. Ross Hearn, Debbie Rice, Alison E. Mather, Julian Parkhill, Sara J. Brown, Charlotte M. Proby, Matthew T. G. Holden

School/Research organisations

Abstract

Staphylococcus aureus is an opportunistic pathogen and variable component of the human microbiota. In atopic eczema (AE) a characteristic of the disease is colonization by S. aureus, with exacerbations associated with an increased bacterial burden of the organism. Despite this, the origins and genetic diversity of S. aureus colonizing individual patients during AE disease flares is poorly understood. To examine the micro-evolution of S. aureus colonization we have deep-sequenced S. aureus populations from nine children with moderate to severe AE, and 18 non-atopic children asymptomatically carrying S. aureus nasally. Colonization by clonal S. aureus populations was observed in both AE cases and controls, with all but one of the individuals containing colonies belonging to a single sequence type. Phylogenetic analysis revealed that disease flares were associated with the clonal expansion of the S. aureus population, occurring over a period of weeks to months. There was a significant difference in the genetic backgrounds of S. aureus colonizing AE patients versus controls (Fisher’s Exact test, p=0.03). Examination of intra-host genetic heterogeneity of the colonizing S. aureus populations identified evidence of within-host selection in the AE patients, with AE variants being potentially selectively advantageous for intracellular persistence and treatment resistance.
Close

Details

Original languageEnglish
Pages (from-to)336-343
JournalJournal of Investigative Dermatology
Volume138
Issue number2
Early online date23 Sep 2017
DOIs
Publication statusPublished - Feb 2018

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Draft genome sequences of the type strains of Actinobacillus indolicus (46K2C) and Actinobacillus porcinus (NM319), two NAD-dependent bacterial species found in the respiratory tract of pigs

    Bossé, J. T., Li, Y., Fernandez Crespo, R., Angen, Ø., Holden, M. T. G., Weinert, L. A., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R. & BRaDP1T Consortium, 2 Jan 2020, In : Microbiology Resource Announcements. 9, 3 p., e00716-19.

    Research output: Contribution to journalArticle

  2. The emergence of successful Streptococcus pyogenes lineages through convergent pathways of capsule loss and recombination directing high toxin expression

    Turner, C. E., Holden, M. T. G., Blane, B., Horner, C., Peacock, S. J. & Sriskandan, S., 10 Dec 2019, In : mBio. 10, 6, p. 1-19 20 p., e02521.

    Research output: Contribution to journalArticle

  3. Evolution and global transmission of a multidrug-resistant, community-associated methicillin-resistant Staphylococcus aureus lineage from the Indian subcontinent

    Steinig, E. J., Duchene, S., Robinson, D. A., Monecke, S., Yokoyama, M., Laabei, M., Slickers, P., Andersson, P., Williamson, D., Kearns, A., Goering, R. V., Dickson, E., Ehricht, R., Ip, M., O'Sullivan, M. V. N., Coombs, G. W., Petersen, A., Brennan, G., Shore, A. C., Coleman, D. C. & 18 others, Pantosti, A., de Lencastre, H., Westh, H., Kobayashi, N., Heffernan, H., Strommenger, B., Layer, F., Weber, S., Aamot, H. V., Skakni, L., Peacock, S. J., Sarovich, D., Harris, S., Parkhill, J., Massey, R. C., Holden, M. T. G., Bentley, S. D. & Tong, S. Y. C., 26 Nov 2019, In : mBio. 10, 6, p. 1-20 20 p., e01105.

    Research output: Contribution to journalArticle

  4. Genetic variation associated with infection and the environment in the accidental pathogen Burkholderia pseudomallei

    Chewapreecha, C., Mather, A. E., Harris, S. R., Hunt, M., Holden, M. T. G., Chaichana, C., Wuthiekanun, V., Dougan, G., Day, N. P. J., Limmathurotsakul, D., Parkhill, J. & Peacock, S. J., 22 Nov 2019, In : Communications Biology. 2, 11 p., 428.

    Research output: Contribution to journalArticle

  5. Improved characterisation of MRSA transmission using within-host bacterial sequence diversity

    Hall, M. D., Holden, M. TG., Srisomang, P., Mahavanakul, W., Wuthiekanun, V., Limmathurotsakul, D., Fountain, K., Parkhill, J., Nickerson, E. K., Peacock, S. J. & Fraser, C., 8 Oct 2019, In : eLife. 8, e46402.

    Research output: Contribution to journalArticle

Related by journal

  1. The genetic diversity of Staphylococcus aureus colonisation in atopic eczema disease flare

    Harkins, C. P., Pettigrew, K. A., Oravcova, K., Gardner, J., Hearn, R., Rice, D., Mather, A. E., Parkhill, J., Brown, S. J., Proby, C. M. & Holden, M. T., Oct 2017, In : Journal of Investigative Dermatology. 137, 10, p. S258-S258 1 p.

    Research output: Contribution to journalAbstract

  2. Pharmacological stimulation of Edar signaling in the adult enhances sebaceous gland size and function

    Kowalczyk-Quintas, C., Schuepbach-Mallepell, S., Willen, L., Smith, T. K., Huttner, K., Kirby, N., Headon, D. J. & Schneider, P., 2015, In : Journal of Investigative Dermatology. 135, 2, p. 359-368 10 p.

    Research output: Contribution to journalArticle

  3. Topically applied nitric oxide induces T-Lymphocyte infiltration in human skin, but minimal inflammation

    Mowbray, M., Tan, X., Wheatley, P. S., Morris, R. E. & Weller, R. B., Feb 2008, In : Journal of Investigative Dermatology. 128, 2, p. 352-360 9 p.

    Research output: Contribution to journalArticle

  4. Topically applied nitric oxide induces T-lymphocyte infiltration in human skin, but minimal inflammation (vol 128, pg 352, 2008)

    Mowbray, M., Tan, X., Wheatley, P. S., Rossi, A. G., Morris, R. E. & Weller, R. B., Oct 2008, In : Journal of Investigative Dermatology. 128, 10, p. 2546-2546 1 p.

    Research output: Contribution to journalArticle

ID: 251160165

Top