Skip to content

Research at St Andrews

The potato mop-top virus TGB2 protein and viral RNA associate with chloroplasts and viral infection induces inclusions in the plastids

Research output: Contribution to journalArticlepeer-review

DOI

Author(s)

Graham H. Cowan, Alison G. Roberts, Sean N. Chapman, Angelika Ziegler, Eugene I. Savenkov, Lesley Torrance

School/Research organisations

Abstract

The potato mop-top virus (PMTV triple gene block 2 (TGB2 movement proteins fused to monomeric red fluorescent protein (mRFP-TGB2 was expressed under the control of the PMTV subgenomic promoter from a PMTV vector. The subcellular localizations and interactions of mRFP-TGB2 were investigated using confocal imaging [confocal laser-scanning microscope, (CLSM] and biochemical analysis. The results revealed associations with membranes of the endoplasmic reticulum (ER, mobile granules, small round structures (1-2 μm in diameter, and chloroplasts. Expression of mRFP-TGB2 in epidermal cells enabled cell-to-cell movement of a TGB2 defective PMTV reporter clone, indicating that the mRFP-TGB2 fusion protein was functional and required for cell-to-cell movement. Protein-lipid interaction assays revealed an association between TGB2 and lipids present in chloroplasts, consistent with microscopical observations where the plastid envelope was labeled later in infection. To further investigate the association of PMTV infection with chloroplasts, ultrastructural studies of thin sections of PMTV-infected potato and Nicotiana benthamiana leaves by electron microscopy revealed abnormal chloroplasts with cytoplasmic inclusions and terminal projections. Viral coat protein (CP, genomic RNA and fluorescently-labeled TGB2 were detected in plastid preparations isolated from the infected leaves, and viral RNA was localized to chloroplasts in infected tissues. The results reveal a novel association of TGB2 and vRNA with chloroplasts, and suggest viral replication is associated with chloroplast membranes, and that TGB2 plays a novel role in targeting the virus to chloroplasts.

Close

Details

Original languageEnglish
Article number290
JournalFrontiers in Plant Science
Volume3
Issue numberDEC
DOIs
Publication statusPublished - 24 Dec 2012

    Research areas

  • Chloroplasts, Inclusions, PMTV, Replication, TGB2, Viral RNA

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. TERMINAL FLOWER-1/CENTRORADIALIS inhibits tuberization via protein interaction with the tuberigen activation complex

    Zhang, X., Campbell, R., Ducreux, L. J. M., Morris, J., Hedley, P. E., Mellado-Ortega, E., Roberts, A. G., Stephens, J., Bryan, G. J., Torrance, L., Chapman, S. N., Prat, S. & Taylor, M. A., 14 Jul 2020, In: The Plant Journal. Early View, 16 p.

    Research output: Contribution to journalArticlepeer-review

  2. Potato mop-top virus TGB1 co-opts HIPP26 to activate abiotic stress signalling for long distance movement

    Torrance, L., Cowan, G., Roberts, A. G., Jones, S., Kumar, P., Hemsley, P. & Savenkov, E., 2 Aug 2018.

    Research output: Contribution to conferenceAbstractpeer-review

  3. Potato mop-top virus co-opts the stress sensor HIPP26 for long-distance movement

    Cowan, G. H., Roberts, A. G., Jones, S., Kumar, P., Kalyandurg, P. B., Gil, J. F., Savenkov, E. I., Hemsley, P. A. & Torrance, L., 6 Mar 2018, In: Plant Physiology. 176, 3, p. 2052-2070 19 p.

    Research output: Contribution to journalArticlepeer-review

  4. Foresight and trade-off analyses: tools for science strategy development in agriculture and food systems research

    Barrett, C. B., Beaudreault, A. R., Meinke, H., Ash, A., Ghezae, N., Kadiyala, S., Nigussie, M., Smith, A. G. & Torrance, L., 14 Jan 2021, In: Q Open. 1, 1, qoaa002.

    Research output: Contribution to journalArticlepeer-review

  5. Potato virus Y emergence and evolution from the Andes of South America to become a major destructive pathogen of potato and other solanaceous crops worldwide

    Torrance, L. & Talianksy, M. E., 12 Dec 2020, In: Viruses. 12, 12, 14 p., 1430.

    Research output: Contribution to journalReview articlepeer-review

Related by journal

  1. Frontiers in Plant Science (Journal)

    Jens Tilsner (Guest editor)

    20132014

    Activity: Publication peer-review and editorial work typesEditor of research journal

Related by journal

  1. Resisting potato cyst nematodes with resistance

    Gartner, U., Hein, I., Brown, L. H., Chen, X., Mantelin, S., Sharma, S. K., Dandurand, L-M., Kuhl, J. C., Jones, J. T., Bryan, G. J. & Blok, V. C., 25 Mar 2021, In: Frontiers in Plant Science. 12, 18 p., 661194.

    Research output: Contribution to journalReview articlepeer-review

  2. Nicotiana benthamiana as a transient expression host to produce auxin analogs

    Davis, K., Gkotsi, D. S., Smith, D. R. M., Goss, R. J. M., Caputi, L. & O’Connor, S. E., 20 Nov 2020, In: Frontiers in Plant Science. 11, 9 p., 581675.

    Research output: Contribution to journalArticlepeer-review

  3. Creating contacts between replication and movement at plasmodesmata – a role for membrane contact sites in plant virus infections?

    Levy, A. & Tilsner, J., 3 Jul 2020, In: Frontiers in Plant Science. 11, 8 p., 862.

    Research output: Contribution to journalReview articlepeer-review

  4. Efficient detection of long dsRNA in vitro and in vivo using the dsRNA binding domain from FHV B2 protein

    Monsion, B., Incarbone, M., Hleibieh, K., Poignavent, V., Ghannam, A., Dunoyer, P., Daeffler, L., Tilsner, J. & Ritzenthaler, C., 1 Feb 2018, In: Frontiers in Plant Science. 9, 16 p., 70.

    Research output: Contribution to journalArticlepeer-review

  5. The Globodera pallida SPRYSEC effector GpSPRY-414-2 that suppresses plant defenses targets a regulatory component of the dynamic microtubule network

    Mei, Y., Wright, K. M., Haegeman, A., Bauters, L., Diaz-Granados, A., Goverse, A., Gheysen, G., Jones, J. T. & Mantelin, S., 12 Jul 2018, In: Frontiers in Plant Science. 9, 16 p., 1019.

    Research output: Contribution to journalArticlepeer-review

ID: 255454870

Top