Skip to content

Research at St Andrews

Tools for detection of Mycoplasma amphoriforme: a primary respiratory pathogen?

Research output: Contribution to journalArticle

DOI

Open Access permissions

Open

Author(s)

Clare L. Ling, Katarina Oravcova, Thomas Ferris Beattie, Dean D. Creer, Paul Dilworth, Naomi L. Fulton, Alison Hardie, Michelle Munro, Marcus Pond, Kate Templeton, David Webster, Sarita Workman, Timothy D. McHugh, Stephen Henry Gillespie

School/Research organisations

Abstract

Mycoplasma amphoriforme is a recently described organism isolated from the respiratory tracts of patients with immunodeficiency and evidence of chronic infection. Novel assays for the molecular detection of the organism by real-time quantitative PCRs (qPCRs) targeting the uracil DNA glycosylase gene (udg) or the 23S rRNA gene are described here. The analytical sensitivities are similar to the existing conventional M. amphoriforme 16S rRNA gene PCR, with the advantage of being species specific, rapid, and quantitative. By using these techniques, we demonstrate the presence of this organism in 17 (19.3%) primary antibody-deficient (PAD) patients, 4 (5%) adults with lower respiratory tract infection, 1 (2.6%) sputum sample from a patient attending a chest clinic, and 23 (0.21%) samples submitted for viral diagnosis of respiratory infection, but not in normal adult control subjects. These data show the presence of this microorganism in respiratory patients and suggest that M. amphoriforme may infect both immunocompetent and immunocompromised people. Further studies to characterize this organism are required, and this report provides the tools that may be used by other research groups to investigate its pathogenic potential.

Close

Details

Original languageEnglish
Pages (from-to)1177-1181
Number of pages5
JournalJournal of Clinical Microbiology
Volume52
Issue number4
Early online date1 Apr 2014
DOIs
Publication statusPublished - Apr 2014

    Research areas

  • Mycoplasma, Pathogen discover, Lower respiratory tract infection

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Mimicking in-vivo exposures to drug combinations in-vitro: anti-tuberculosis drugs in lung lesions and the hollow fiber model of infection

    Kloprogge, F., Hammond, R., Kipper, K., Gillespie, S. H. & Della Pasqua, O., 13 Sep 2019, In : Scientific Reports. 9, 8 p., 13228.

    Research output: Contribution to journalArticle

  2. A tuberculosis molecular bacterial load assay (TB-MBLA)

    Sabiiti, W., Mtafya, B. A., Alferes De Lima, D., Dombay, E., Baron, V. O., Azam, K., Orascova, K., Sloan, D. J. & Gillespie, S. H., 6 Sep 2019, (Accepted/In press) In : Journal of Visualized Experiments.

    Research output: Contribution to journalArticle

  3. Can phenotypic data complement our understanding of antimycobacterial effects for drug combinations?

    Kloprogge, F., Hammond, R., Copas, A., Gillespie, S. H. & Della Pasqua, O., 25 Aug 2019, In : Journal of Antimicrobial Chemotherapy. Advance article, 7 p.

    Research output: Contribution to journalArticle

  4. Toxicity related to standard TB therapy for pulmonary tuberculosis and treatment outcomes in the REMoxTB study according to HIV status

    Tweed, C. D., Crook, A. M., Dawson, R., Diacon, A. H., McHugh, T. D., Mendel, C. M., Meredith, S. K., Mohapi, L., Murphy, M. E., Nunn, A. J., Phillips, P. P. J., Singh, K. P., Spigelman, M. & Gillespie, S. H., 14 Aug 2019, In : BMC Pulmonary Medicine. 19, 9 p., 152.

    Research output: Contribution to journalArticle

  5. Model-based relationship between the molecular bacterial load assay and time-to-positivity in liquid culture

    Svensson, R. J., Sabiiti, W., Kibiki, G. S., Ntinginya, N. E., Bhatt, N., Davies, G., Gillespie, S. H. & Simonsson, U. S. H., 29 Jul 2019, In : Antimicrobial Agents and Chemotherapy. Early

    Research output: Contribution to journalArticle

Related by journal

  1. Heat-inactivation renders sputum safe and preserves Mycobacterium tuberculosis RNA for downstream molecular tests

    Sabiiti, W., Azam, K., Esmeraldo, E., Bhatt, N., Rachow, A. & Gillespie, S. H., Mar 2019, In : Journal of Clinical Microbiology. 57, 4, 8 p., e01778-18.

    Research output: Contribution to journalArticle

  2. Molecular bacterial load assay (MBLA) concurs with culture on the NaOH-induced Mycobacterium tuberculosis loss of viability

    Mtafya, B., Sabiiti, W., Sabi, I., John, J., Sichone, E., Ntinginya, N. E. & Gillespie, S. H., 25 Jun 2019, In : Journal of Clinical Microbiology. 57, e01992-18.

    Research output: Contribution to journalArticle

  3. Development of a multilocus sequence typing scheme for the molecular typing of Mycoplasma pneumoniae

    Brown, R. J., Holden, M., Spiller, O. B. & Chalker, V. J., Oct 2015, In : Journal of Clinical Microbiology. 53, 10, p. 3195-3203

    Research output: Contribution to journalArticle

  4. Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy

    De Smet, B., Sarovich, D. S., Price, E. P., Mayo, M., Theobald, V., Kham, C., Heng, S., Phe, T., Holden, M. T. G., Parkhill, J., Peacock, S. J., Spratt, B. G., Jacobs, J., Vandamme, P. & Currie, B. J., Jan 2015, In : Journal of Clinical Microbiology. 53, 1, p. 323-326

    Research output: Contribution to journalArticle

  5. Micro-evolution of Burkholderia pseudomallei during an acute infection

    Limmathurotsakul, D., Holden, M. T. G., Coupland, P., Price, E. P., Chantratita, N., Wuthiekanun, V., Amornchai, P., Parkhill, J. & Peacock, S. J., 2014, In : Journal of Clinical Microbiology.

    Research output: Contribution to journalArticle

ID: 138259004