Skip to content

Research at St Andrews

TRIC-B channels display labile gating: evidence from the TRIC-A knockout mouse model

Research output: Contribution to journalArticle


Elisa Venturi, Antoni Matyjaszkiewicz, Samantha Jane Pitt, Krasimira Tsaneva-Atanasova, Miyuki Nishi, Daiju Yamazaki, Hiroshi Takeshima, Rebecca Sitsapesan

School/Research organisations


Sarcoplasmic/endoplasmic reticulum (SR) and nuclear membranes contain two related cation channels named TRIC-A and TRIC-B. In many tissues, both subtypes are co-expressed, making it impossible to distinguish the distinct single-channel properties of each subtype. We therefore incorporated skeletal muscle SR vesicles derived from Tric-a-knockout mice into bilayers in order to characterise the biophysical properties of native TRIC-B without possible misclassification of the channels as TRIC-A, and without potential distortion of functional properties by detergent purification protocols. The native TRIC-B channels were ideally selective for cations. In symmetrical 210 mM K+, the maximum (full) open channel level (199 pS) was equivalent to that observed when wild-type SR vesicles were incorporated into bilayers. Analysis of TRIC-B gating revealed complex and variable behaviour. Four main sub-conductance levels were observed at approximately 80 % (161 pS), 60 % (123 pS), 46 % (93 pS), and 30 % (60 pS) of the full open state. Seventy-five percent of the channels were voltage sensitive with Po being markedly reduced at negative holding potentials. The frequent, rapid transitions between TRIC-B sub-conductance states prevented development of reliable gating models using conventional single-channel analysis. Instead, we used mean-variance plots to highlight key features of TRIC-B gating in a more accurate and visually useful manner. Our study provides the first biophysical characterisation of native TRIC-B channels and indicates that this channel would be suited to provide counter current in response to Ca2+ release from the SR. Further experiments are required to distinguish the distinct functional properties of TRIC-A and TRIC-B and understand their individual but complementary physiological roles.



Original languageEnglish
Pages (from-to)1135-1148
JournalPflügers Archiv: European Journal of Physiology
Issue number8
Publication statusPublished - Aug 2013

    Research areas

  • Calcium-release, Ion channel, Excitation-contraction coupling, Sarcoplasmic reticulum

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Total plasma magnesium, zinc, copper and selenium concentrations in type-I and type-II diabetes

    Sobczak, A. I. S., Stefanowicz, F., Pitt, S. J., Ajjan, R. A. & Stewart, A. J., Feb 2019, In : BioMetals. 32, 1, p. 123-138 16 p.

    Research output: Contribution to journalArticle

  2. Influence of zinc on glycosaminoglycan neutralisation during coagulation

    Sobczak, A. I. S., Pitt, S. J. & Stewart, A. J., Sep 2018, In : Metallomics. 10, 9, p. 1180-1190 11 p.

    Research output: Contribution to journalReview article

  3. Glycosaminoglycan neutralization in coagulation control

    Sobczak, A. I. S., Pitt, S. J. & Stewart, A. J., Jun 2018, In : Arteriosclerosis, Thrombosis, and Vascular Biology. 38, 6, p. 1258-1270 14 p.

    Research output: Contribution to journalReview article

  4. Modulation of the Neisseria gonorrhoeae drug efflux conduit MtrE

    Tamburrino, G., Llabrés, S., Vickery, O., Pitt, S. J. & Zachariae, U., 6 Dec 2017, In : Scientific Reports. 7, 10 p., 17091.

    Research output: Contribution to journalArticle

  5. Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high fat diet

    Jovanovic, A., Obradovic, M., Sudar-Milovanovic, E., Stewart, A. J., Pitt, S. J., Alvantic, D., Aleksic, E. & Isenovic, E. R., Dec 2017, In : Molecular and Cellular Biochemistry. 436, 1-2, p. 49-58 10 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Activity modes and modulation of the peptide-gated Na(+) channel of Helix neurones

    Green, KA. & Cottrell, G. A., Mar 2002, In : Pflügers Archiv: European Journal of Physiology. 443, 5-6, p. 813-821 9 p.

    Research output: Contribution to journalArticle

  2. Modulation of ligand-gated dopamine channels in Helix neurones

    Cottrell, G. A. & Green, KA., Jun 1997, In : Pflügers Archiv: European Journal of Physiology. 434, 3, p. 313-322 10 p.

    Research output: Contribution to journalArticle

ID: 48756481