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Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation

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Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation. / Hulme, Charlotte H.; Wilson, Emma L.; Fuller, Heidi R.; Roberts, Sally; Richardson, James B.; Gallacher, Pete; Peffers, Mandy J.; Shirran, Sally L.; Botting, Catherine H.; Wright, Karina T.

In: Arthritis Research & Therapy, Vol. 20, 87, 02.05.2018.

Research output: Contribution to journalArticle

Harvard

Hulme, CH, Wilson, EL, Fuller, HR, Roberts, S, Richardson, JB, Gallacher, P, Peffers, MJ, Shirran, SL, Botting, CH & Wright, KT 2018, 'Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation', Arthritis Research & Therapy, vol. 20, 87. https://doi.org/10.1186/s13075-018-1573-4

APA

Hulme, C. H., Wilson, E. L., Fuller, H. R., Roberts, S., Richardson, J. B., Gallacher, P., ... Wright, K. T. (2018). Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation. Arthritis Research & Therapy, 20, [87]. https://doi.org/10.1186/s13075-018-1573-4

Vancouver

Hulme CH, Wilson EL, Fuller HR, Roberts S, Richardson JB, Gallacher P et al. Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation. Arthritis Research & Therapy. 2018 May 2;20. 87. https://doi.org/10.1186/s13075-018-1573-4

Author

Hulme, Charlotte H. ; Wilson, Emma L. ; Fuller, Heidi R. ; Roberts, Sally ; Richardson, James B. ; Gallacher, Pete ; Peffers, Mandy J. ; Shirran, Sally L. ; Botting, Catherine H. ; Wright, Karina T. / Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation. In: Arthritis Research & Therapy. 2018 ; Vol. 20.

Bibtex - Download

@article{9a8a9986d68848489b2727b98fc8d740,
title = "Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation",
abstract = "Autologous chondrocyte implantation (ACI) has a failure rate of approximately 20{\%}, but it is yet to be fully understood why. Biomarkers are needed that can pre-operatively predict in which patients it is likely to fail, so that alternative or individualised therapies can be offered. We previously used label-free quantitation (LF) with a dynamic range compression proteomic approach to assess the synovial fluid (SF) of ACI responders and non-responders. However, we were able to identify only a few differentially abundant proteins at baseline. In the present study, we built upon these previous findings by assessing higher-abundance proteins within this SF, providing a more global proteomic analysis on the basis of which more of the biology underlying ACI success or failure can be understood.",
keywords = "Autologous chondrocyte implantation (ACI), iTRAQ proteomics, Label-free quantitation proteomics, Synovial fluid, Cartilage repair, Complement C1S subcomponent, Matrix metalloproteinase 3, MMP3",
author = "Hulme, {Charlotte H.} and Wilson, {Emma L.} and Fuller, {Heidi R.} and Sally Roberts and Richardson, {James B.} and Pete Gallacher and Peffers, {Mandy J.} and Shirran, {Sally L.} and Botting, {Catherine H.} and Wright, {Karina T.}",
note = "We thank Arthritis Research UK for supporting this work via grants 19429, 20815 and 21122. MJP is supported through a Wellcome Trust Clinical Intermediate Fellowship. This work was supported by Wellcome Trust grant 094476/Z/10/Z, which funded the purchase of the TripleTOF 5600 mass spectrometer at the Biomedical Sciences Research Complex Mass Spectrometry and Proteomics Facility, University of St. Andrews (Fife, UK).",
year = "2018",
month = "5",
day = "2",
doi = "10.1186/s13075-018-1573-4",
language = "English",
volume = "20",
journal = "Arthritis Research & Therapy",
issn = "1478-6354",
publisher = "BioMed Central",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation

AU - Hulme, Charlotte H.

AU - Wilson, Emma L.

AU - Fuller, Heidi R.

AU - Roberts, Sally

AU - Richardson, James B.

AU - Gallacher, Pete

AU - Peffers, Mandy J.

AU - Shirran, Sally L.

AU - Botting, Catherine H.

AU - Wright, Karina T.

N1 - We thank Arthritis Research UK for supporting this work via grants 19429, 20815 and 21122. MJP is supported through a Wellcome Trust Clinical Intermediate Fellowship. This work was supported by Wellcome Trust grant 094476/Z/10/Z, which funded the purchase of the TripleTOF 5600 mass spectrometer at the Biomedical Sciences Research Complex Mass Spectrometry and Proteomics Facility, University of St. Andrews (Fife, UK).

PY - 2018/5/2

Y1 - 2018/5/2

N2 - Autologous chondrocyte implantation (ACI) has a failure rate of approximately 20%, but it is yet to be fully understood why. Biomarkers are needed that can pre-operatively predict in which patients it is likely to fail, so that alternative or individualised therapies can be offered. We previously used label-free quantitation (LF) with a dynamic range compression proteomic approach to assess the synovial fluid (SF) of ACI responders and non-responders. However, we were able to identify only a few differentially abundant proteins at baseline. In the present study, we built upon these previous findings by assessing higher-abundance proteins within this SF, providing a more global proteomic analysis on the basis of which more of the biology underlying ACI success or failure can be understood.

AB - Autologous chondrocyte implantation (ACI) has a failure rate of approximately 20%, but it is yet to be fully understood why. Biomarkers are needed that can pre-operatively predict in which patients it is likely to fail, so that alternative or individualised therapies can be offered. We previously used label-free quantitation (LF) with a dynamic range compression proteomic approach to assess the synovial fluid (SF) of ACI responders and non-responders. However, we were able to identify only a few differentially abundant proteins at baseline. In the present study, we built upon these previous findings by assessing higher-abundance proteins within this SF, providing a more global proteomic analysis on the basis of which more of the biology underlying ACI success or failure can be understood.

KW - Autologous chondrocyte implantation (ACI)

KW - iTRAQ proteomics

KW - Label-free quantitation proteomics

KW - Synovial fluid

KW - Cartilage repair

KW - Complement C1S subcomponent

KW - Matrix metalloproteinase 3

KW - MMP3

U2 - 10.1186/s13075-018-1573-4

DO - 10.1186/s13075-018-1573-4

M3 - Article

VL - 20

JO - Arthritis Research & Therapy

JF - Arthritis Research & Therapy

SN - 1478-6354

M1 - 87

ER -

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