Skip to content

Research at St Andrews

Using blubber explants to investigate adipose function in grey seals: glycolytic, lipolytic and gene expression responses to glucose and hydrocortisone

Research output: Contribution to journalArticle

Author(s)

Kimberley A. Bennett, Kelly J. Robinson, Simon E. W. Moss, Sebastian Millward, Ailsa J. Hall

School/Research organisations

Abstract

Adipose tissue is fundamental to energy balance, which underpins fitness and survival. Knowledge of adipose regulation in animals that undergo rapid fat deposition and mobilisation aids understanding of their energetic responses to rapid environmental change. Tissue explants can be used to investigate adipose regulation in wildlife species with large fat reserves, when opportunities for organismal experimental work are limited. We investigated glucose removal, lactate, glycerol and NEFA accumulation in media, and metabolic gene expression in blubber explants from wild grey seals. Glycolysis was higher in explants incubated in 25 mM glucose (HG) for 24 h compared to controls (C: 5.5 mM glucose). Adipose-derived lactate likely contributes to high endogenous glucose production in seals. Lipolysis was not stimulated by HG or high hydrocortisone (HC: 500 nM hydrocortisone) and was lower in heavier animals. HC caused NEFA accumulation in media to decrease by ~30% relative to C in females, indicative of increased lipogenesis. Lipolysis was higher in males than females in C and HG conditions. Lower relative abundance of 11-β-hydroxysteroid dehydrogenase 1 mRNA in HG explants suggests glucose involvement in blubber cortisol sensitivity. Our findings can help predict energy balance responses to stress and nutritional state in seals, and highlight the use of explants to study fat tissue function in wildlife.
Close

Details

Original languageEnglish
Article number7731
Number of pages11
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 10 Aug 2017

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Mercury in cetaceans: exposure, bioaccumulation and toxicity

    Kershaw, J. & Hall, A., Dec 2019, In : Science of the Total Environment. 694, p. 1-11 11 p., 133683.

    Research output: Contribution to journalReview article

  2. Viral emergence in marine mammals in the North Pacific may be linked to Arctic sea ice reduction

    VanWormer, E., Mazet, J. A. K., Hall, A., Gill, V. A., Boveng, P. L., London, J. M., Gelatt, T., Fadely, B. S., Lander, M. E., Sterling, J., Burkanov, V. N., Ream, R. R., Brock, P. M., Rea, L. D., Smith, B. R., Jeffers, A., Henstock, M., Rehberg, M. J., Burek-Huntington, K. A., Cosby, S. L. & 2 others, Hammond, J. A. & Goldstein, T., 7 Nov 2019, In : Scientific Reports. 9, 11 p., 15569.

    Research output: Contribution to journalArticle

  3. Age–length relationships in UK harbour seals during a period of population decline

    Hall, A. J., Mackey, B., Kershaw, J. L. & Thompson, P., 6 Sep 2019, In : Aquatic Conservation: Marine and Freshwater Ecosystems. 29, S1, p. 61-70 10 p.

    Research output: Contribution to journalArticle

  4. Investigating decadal changes in persistent organic pollutants in Scottish grey seal pups

    Robinson, K. J., Hall, A. J., Scholl, G., Debier, C., Thomé, J-P., Eppe, G., Adam, C. & Bennett, K. A., 6 Sep 2019, In : Aquatic Conservation: Marine and Freshwater Ecosystems. 29, S1, p. 86-100 15 p.

    Research output: Contribution to journalArticle

  5. Assessing cetacean body condition: is total lipid content in blubber biopsies a useful monitoring tool?

    Kershaw, J. L., Brownlow, A., Ramp, C. A., Miller, P. J. O. & Hall, A. J., 6 Sep 2019, In : Aquatic Conservation: Marine and Freshwater Ecosystems. 29, S1, p. 271-282 12 p.

    Research output: Contribution to journalArticle

Related by journal

  1. Scientific Reports (Journal)

    Alan J. Stewart (Member of editorial board)
    Jun 2019 → …

    Activity: Publication peer-review and editorial work typesEditor of research journal

  2. Scientific Reports (Journal)

    Monica Arso Civil (Reviewer)
    Jul 2018 → …

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  3. Scientific Reports (Journal)

    Anouk Margaretha Borst (Reviewer)
    10 Feb 2017

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

  4. Scientific Reports (Journal)

    Emma Louise Carroll (Member of editorial board)
    3 Oct 2016

    Activity: Publication peer-review and editorial work typesEditor of research journal

  5. Scientific Reports (Journal)

    Uli Schwarz-Linek (Reviewer)
    11 May 2016

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

Related by journal

  1. A comparative analysis of response times shows that multisensory benefits and interactions are not equivalent

    Innes, B. R. & Otto, T. U., 27 Feb 2019, In : Scientific Reports. 9, 10 p., 2921.

    Research output: Contribution to journalArticle

  2. A large explosive silicic eruption in the British Palaeogene Igneous Province

    Troll, V. R., Emeleus, C. H., Nicoll, G. R., Mattsson, T., Ellam, R. M., Donaldson, C. H. & Harris, C., 24 Jan 2019, In : Scientific Reports. 9, 1, 15 p., 494.

    Research output: Contribution to journalArticle

  3. An Escherichia coli ST131 pangenome atlas reveals population structure and evolution across 4,071 isolates

    Decano, A. G. & Downing, T., 22 Nov 2019, In : Scientific Reports. 9, 13 p., 17394.

    Research output: Contribution to journalArticle

  4. Automated tumour budding quantification by machine learning augments TNM staging in muscle-invasive bladder cancer prognosis

    Brieu, N., Gavriel, C., Nearchou, I. P., Harrison, D. J., Schmidt, G. & Caie, P. D., 26 Mar 2019, In : Scientific Reports. 9, 5174.

    Research output: Contribution to journalArticle

  5. Balanced cholinergic modulation of spinal locomotor circuits via M2 and M3 muscarinic receptors

    Nascimento, F., Spindler, L. R. B. & Miles, G. B., 1 Oct 2019, In : Scientific Reports. 9, 16 p., 14051.

    Research output: Contribution to journalArticle

ID: 250202795

Top