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Whole-genome sequencing reveals clonal expansion of multiresistant Staphylococcus haemolyticus in European hospitals

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Whole-genome sequencing reveals clonal expansion of multiresistant Staphylococcus haemolyticus in European hospitals. / Cavanagh, Jorunn Pauline; Hjerde, Erik; Holden, Matthew T G; Kahlke, Tim; Klingenberg, Claus; Flægstad, Trond; Parkhill, Julian; Bentley, Stephen D; Sollid, Johanna U Ericson.

In: Journal of Antimicrobial Chemotherapy, Vol. 69, No. 11, 11.11.2014, p. 2920-2927.

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Cavanagh, JP, Hjerde, E, Holden, MTG, Kahlke, T, Klingenberg, C, Flægstad, T, Parkhill, J, Bentley, SD & Sollid, JUE 2014, 'Whole-genome sequencing reveals clonal expansion of multiresistant Staphylococcus haemolyticus in European hospitals', Journal of Antimicrobial Chemotherapy, vol. 69, no. 11, pp. 2920-2927. https://doi.org/10.1093/jac/dku271

APA

Cavanagh, J. P., Hjerde, E., Holden, M. T. G., Kahlke, T., Klingenberg, C., Flægstad, T., ... Sollid, J. U. E. (2014). Whole-genome sequencing reveals clonal expansion of multiresistant Staphylococcus haemolyticus in European hospitals. Journal of Antimicrobial Chemotherapy, 69(11), 2920-2927. https://doi.org/10.1093/jac/dku271

Vancouver

Cavanagh JP, Hjerde E, Holden MTG, Kahlke T, Klingenberg C, Flægstad T et al. Whole-genome sequencing reveals clonal expansion of multiresistant Staphylococcus haemolyticus in European hospitals. Journal of Antimicrobial Chemotherapy. 2014 Nov 11;69(11):2920-2927. https://doi.org/10.1093/jac/dku271

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Cavanagh, Jorunn Pauline ; Hjerde, Erik ; Holden, Matthew T G ; Kahlke, Tim ; Klingenberg, Claus ; Flægstad, Trond ; Parkhill, Julian ; Bentley, Stephen D ; Sollid, Johanna U Ericson. / Whole-genome sequencing reveals clonal expansion of multiresistant Staphylococcus haemolyticus in European hospitals. In: Journal of Antimicrobial Chemotherapy. 2014 ; Vol. 69, No. 11. pp. 2920-2927.

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@article{bf8f05d0fb9443ffb099812031b75274,
title = "Whole-genome sequencing reveals clonal expansion of multiresistant Staphylococcus haemolyticus in European hospitals",
abstract = "Objectives Staphylococcus haemolyticus is an emerging cause of nosocomial infections, primarily affecting immunocompromised patients. A comparative genomic analysis was performed on clinical S. haemolyticus isolates to investigate their genetic relationship and explore the coding sequences with respect to antimicrobial resistance determinants and putative hospital adaptation.Methods Whole-genome sequencing was performed on 134 isolates of S. haemolyticus from geographically diverse origins (Belgium, 2; Germany, 10; Japan, 13; Norway, 54; Spain, 2; Switzerland, 43; UK, 9; USA, 1). Each genome was individually assembled. Protein coding sequences (CDSs) were predicted and homologous genes were categorized into three types: Type I, core genes, homologues present in all strains; Type II, unique core genes, homologues shared by only a subgroup of strains; and Type III, unique genes, strain-specific CDSs. The phylogenetic relationship between the isolates was built from variable sites in the form of single nucleotide polymorphisms (SNPs) in the core genome and used to construct a maximum likelihood phylogeny.Results SNPs in the genome core regions divided the isolates into one major group of 126 isolates and one minor group of isolates with highly diverse genomes. The major group was further subdivided into seven clades (A–G), of which four (A–D) encompassed isolates only from Europe. Antimicrobial multiresistance was observed in 77.7{\%} of the collection. High levels of homologous recombination were detected in genes involved in adherence, staphylococcal host adaptation and bacterial cell communication.Conclusions The presence of several successful and highly resistant clones underlines the adaptive potential of this opportunistic pathogen.",
keywords = "Staphylococci, SCCmec, Molecular epidemiology, Multidrug resistance, Bacterial genomics",
author = "Cavanagh, {Jorunn Pauline} and Erik Hjerde and Holden, {Matthew T G} and Tim Kahlke and Claus Klingenberg and Trond Fl{\ae}gstad and Julian Parkhill and Bentley, {Stephen D} and Sollid, {Johanna U Ericson}",
year = "2014",
month = "11",
day = "11",
doi = "10.1093/jac/dku271",
language = "English",
volume = "69",
pages = "2920--2927",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "11",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Whole-genome sequencing reveals clonal expansion of multiresistant Staphylococcus haemolyticus in European hospitals

AU - Cavanagh, Jorunn Pauline

AU - Hjerde, Erik

AU - Holden, Matthew T G

AU - Kahlke, Tim

AU - Klingenberg, Claus

AU - Flægstad, Trond

AU - Parkhill, Julian

AU - Bentley, Stephen D

AU - Sollid, Johanna U Ericson

PY - 2014/11/11

Y1 - 2014/11/11

N2 - Objectives Staphylococcus haemolyticus is an emerging cause of nosocomial infections, primarily affecting immunocompromised patients. A comparative genomic analysis was performed on clinical S. haemolyticus isolates to investigate their genetic relationship and explore the coding sequences with respect to antimicrobial resistance determinants and putative hospital adaptation.Methods Whole-genome sequencing was performed on 134 isolates of S. haemolyticus from geographically diverse origins (Belgium, 2; Germany, 10; Japan, 13; Norway, 54; Spain, 2; Switzerland, 43; UK, 9; USA, 1). Each genome was individually assembled. Protein coding sequences (CDSs) were predicted and homologous genes were categorized into three types: Type I, core genes, homologues present in all strains; Type II, unique core genes, homologues shared by only a subgroup of strains; and Type III, unique genes, strain-specific CDSs. The phylogenetic relationship between the isolates was built from variable sites in the form of single nucleotide polymorphisms (SNPs) in the core genome and used to construct a maximum likelihood phylogeny.Results SNPs in the genome core regions divided the isolates into one major group of 126 isolates and one minor group of isolates with highly diverse genomes. The major group was further subdivided into seven clades (A–G), of which four (A–D) encompassed isolates only from Europe. Antimicrobial multiresistance was observed in 77.7% of the collection. High levels of homologous recombination were detected in genes involved in adherence, staphylococcal host adaptation and bacterial cell communication.Conclusions The presence of several successful and highly resistant clones underlines the adaptive potential of this opportunistic pathogen.

AB - Objectives Staphylococcus haemolyticus is an emerging cause of nosocomial infections, primarily affecting immunocompromised patients. A comparative genomic analysis was performed on clinical S. haemolyticus isolates to investigate their genetic relationship and explore the coding sequences with respect to antimicrobial resistance determinants and putative hospital adaptation.Methods Whole-genome sequencing was performed on 134 isolates of S. haemolyticus from geographically diverse origins (Belgium, 2; Germany, 10; Japan, 13; Norway, 54; Spain, 2; Switzerland, 43; UK, 9; USA, 1). Each genome was individually assembled. Protein coding sequences (CDSs) were predicted and homologous genes were categorized into three types: Type I, core genes, homologues present in all strains; Type II, unique core genes, homologues shared by only a subgroup of strains; and Type III, unique genes, strain-specific CDSs. The phylogenetic relationship between the isolates was built from variable sites in the form of single nucleotide polymorphisms (SNPs) in the core genome and used to construct a maximum likelihood phylogeny.Results SNPs in the genome core regions divided the isolates into one major group of 126 isolates and one minor group of isolates with highly diverse genomes. The major group was further subdivided into seven clades (A–G), of which four (A–D) encompassed isolates only from Europe. Antimicrobial multiresistance was observed in 77.7% of the collection. High levels of homologous recombination were detected in genes involved in adherence, staphylococcal host adaptation and bacterial cell communication.Conclusions The presence of several successful and highly resistant clones underlines the adaptive potential of this opportunistic pathogen.

KW - Staphylococci

KW - SCCmec

KW - Molecular epidemiology

KW - Multidrug resistance

KW - Bacterial genomics

UR - http://jac.oxfordjournals.org/content/69/11/2920/suppl/DC1

U2 - 10.1093/jac/dku271

DO - 10.1093/jac/dku271

M3 - Article

VL - 69

SP - 2920

EP - 2927

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 11

ER -

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