Skip to content

Research at St Andrews

Wide-field multiphoton imaging with TRAFIX

Research output: Chapter in Book/Report/Conference proceedingConference contribution

DOI

Open Access permissions

Open

Abstract

Optical approaches have broadened their impact in recent years with innovations in both wide-field and super-resolution imaging, which now underpin biological and medical sciences. Whilst these advances have been remarkable, to date, the ongoing challenge in optical imaging is to penetrate deeper. TRAFIX is an innovative approach that combines temporal focusing illumination with single-pixel detection to obtain wide-field multiphoton images of fluorescent microscopic samples deep through scattering media without correction. It has been shown that it can image through biological samples such as rat brain or human colon tissue up to a depth of seven scattering mean-free-path lengths. Comparisons of TRAFIX with standard point-scanning two-photon microscopy show that the former can yield a five-fold higher signal-to-background ratio while signifcantly reducing photo bleaching of the specimen. Here, we show the first preliminary demonstration of TRAFIX with three-photon excitation imaging dielectric beads. We discuss the advantages of the TRAFIX approach combined with compressive sensing for biomedicine.
Close

Details

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XIX
EditorsAmmasi Periasamy, Peter T. C. So, Karsten König
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages49
Number of pages9
DOIs
Publication statusPublished - 22 Feb 2019
EventMultiphoton Microscopy in the Biomedical Sciences XIX - The Moscone Center, San Francisco, United States
Duration: 2 Feb 20197 Feb 2019
https://spie.org/PWB/conferencedetails/multiphoton-microscopy?SSO=1

Publication series

NameProceedings of SPIE
PublisherSociety of Photo-optical Instrumentation Engineers
Volume10882
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XIX
Abbreviated titleBIOS
CountryUnited States
CitySan Francisco
Period2/02/197/02/19
Internet address

    Research areas

  • Temporal focusing, Single-pixel imaging, Scattering media, Multiphoton microscopy, Three-photon compressive sensing

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. Optimal compressive multiphoton imaging at depth using single-pixel detection

    Wijesinghe, P., Escobet Montalban, A., Chen, M., Munro, P. R. T. & Dholakia, K., 15 Oct 2019, In : Optics Letters. 44, 20, p. 4981-4984 4 p.

    Research output: Contribution to journalLetter

  2. Spatially offset optical coherence tomography: enhancing image contrast at depth

    Chen, M., Wijesinghe, P., Mas Soler, J., Andersen, P. E. & Dholakia, K., 20 Nov 2018, (Submitted) In : Nature Photonics.

    Research output: Contribution to journalArticle

  3. Wide-field multiphoton imaging through scattering media without correction

    Escobet-Montalbán, A., Spesyvtsev, R., Chen, M., Saber, W. A., Andrews, M., Herrington, C. S., Mazilu, M. & Dholakia, K., 12 Oct 2018, In : Science Advances. 4, 10, eaau1338.

    Research output: Contribution to journalArticle

  4. Label-free optical hemogram of granulocytes enhanced by artificial neural networks

    Gupta, R., Chen, M., Malcolm, G. P. A., Hempler, N., Dholakia, K. & Powis, S. J., 13 May 2019, In : Optics Express. 27, 10, p. 13706-13720 15 p.

    Research output: Contribution to journalArticle

  5. Overcoming the speckle correlation limit to achieve a fiber wavemeter with attometer resolution

    Bruce, G. D., O'Donnell, L., Chen, M. & Dholakia, K., 15 Mar 2019, In : Optics Letters. 44, 6, p. 1367-1370

    Research output: Contribution to journalArticle

ID: 257965114

Top