Skip to content

Research at St Andrews

Yeast telomerase and the SUN domain protein Mps3 anchor telomeres and repress subtelomeric recombination

Research output: Research - peer-reviewArticle



Heiko Schober, Helder Casimiro Ferreira, Ronique Kalck, Lutz R. Gehlen, Susan M. Gasser

School/Research organisations


Telomeres form the ends of linear chromosomes and protect these ends from being recognized as DNA double-strand breaks. Telomeric sequences are maintained in most cells by telomerase, a reverse transcriptase that adds TG-rich repeats to chromosome ends. In budding yeast, telomeres are organized in clusters at the nuclear periphery by interactions that depend on components of silent chromatin and the telomerase-binding factor yeast Ku (yKu). In this study, we examined whether the subnuclear localization of telomeres affects end maintenance. A telomere anchoring pathway involving the catalytic yeast telomerase subunits Est2, Est1, and Tlc1 is shown to be necessary for the perinuclear anchoring activity of Yku80 during S phase. Additionally, we identify the conserved Sad1-UNC-84 (SUN) domain protein Mps3 as the principal membrane anchor for this pathway. Impaired interference with Mps3 anchoring through overexpression of the Mps3 N terminus in a tel1 deletion background led to a senescence phenotype and to deleterious levels of subtelomeric Y' recombination. This suggests that telomere binding to the nuclear envelope helps protect telomeric repeats from recombination. Our results provide an example of a specialized structure that requires proper spatiotemporal localization to fulfill its biological role, and identifies a novel pathway of telomere protection.



Original languageEnglish
Pages (from-to)928-938
Number of pages11
JournalGenes & Development
Issue number8
StatePublished - 15 Apr 2009

    Research areas

  • telomerase, Sad1/UNC-84 (SUN) homology domain, CAJAL BODIES, ATM homolog Tel1, Telomere protection, DNA-DAMAGE, ELONGATION, UBIQUITIN LIGASE, nuclear envelope, nuclear organization, RNA, BUDDING YEAST, SACCHAROMYCES-CEREVISIAE, Mps3, REPLICATION, SPINDLE POLE BODY, CHROMOSOME ENDS

Discover related content
Find related publications, people, projects and more using interactive charts.

View graph of relations

Related by author

  1. The shelterin protein POT-1 anchors Caenorhabditis elegans telomeres through SUN-1 at the nuclear periphery

    Ferreira, H. C., Towbin, B. D., Jegou, T. & Gasser, S. M. 9 Dec 2013 In : Journal of Cell Biology. 203, 5, p. 727-735 9 p.

    Research output: Research - peer-reviewArticle

  2. Regulating repression: roles for the Sir4 N-terminus in linker DNA protection and stabilization of epigenetic states

    Kueng, S., Tsai-Pflugfelder, M., Oppikofer, M., Ferreira, H. C., Roberts, E., Tsai, C., Roloff, T-C., Sack, R. & Gasser, S. M. May 2012 In : PLoS Genetics. 8, 5, 19 p., e1002727

    Research output: Research - peer-reviewArticle

  3. The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast

    Ferreira, H. C., Luke, B., Schober, H., Kalck, V., Lingner, J. & Gasser, S. M. Jul 2011 In : Nature Cell Biology. 13, 7, p. 867-874

    Research output: Research - peer-reviewArticle

  4. MacroH2A Allows ATP-Dependent Chromatin Remodeling by SWI/SNF and ACF Complexes but Specifically Reduces Recruitment of SWI/SNF

    Chang, E. Y., Ferreira, H., Somers, J., Nusinow, D. A., Owen-Hughes, T. & Narlikar, G. J. 23 Dec 2008 In : Biochemistry. 47, 51, p. 13726-13732 7 p.

    Research output: Research - peer-reviewArticle

  5. Histone modifications influence the action of Snf2 family remodelling enzymes by different mechanisms

    Ferreira, H., Flaus, A. & Owen-Hughes, T. 30 Nov 2007 In : Journal of Molecular Biology. 374, 3, p. 563-579 17 p.

    Research output: Research - peer-reviewArticle

Related by journal

  1. Identification of a DNA-binding site and transcriptional target for the EWS-WT1(+KTS) oncoprotein

    Reynolds, P. A., Smolen, G. A., Palmer, R. E., Sgroi, D., Yajnik, V., Gerald, W. L. & Haber, D. A. 1 Sep 2003 In : Genes & Development. 17, 17, p. 2094-2107 14 p.

    Research output: Research - peer-reviewArticle

ID: 55082840